PMID- 10406800
OWN - NLM
STAT- MEDLINE
DCOM- 19990902
LR  - 20131121
IS  - 0261-4189 (Print)
IS  - 0261-4189 (Linking)
VI  - 18
IP  - 14
DP  - 1999 Jul 15
TI  - A single amino acid in E-cadherin responsible for host specificity towards the
      human pathogen Listeria monocytogenes.
PG  - 3956-63
AB  - Human E-cadherin promotes entry of the bacterial pathogen Listeria monocytogenes 
      into mammalian cells by interacting with internalin (InlA), a bacterial surface
      protein. Here we show that mouse E-cadherin, although very similar to human
      E-cadherin (85% identity), is not a receptor for internalin. By a series of
      domain-swapping and mutagenesis experiments, we identify Pro16 of E-cadherin as a
      residue critical for specificity: a Pro-->Glu substitution in human E-cadherin
      totally abrogates interaction, whereas a Glu-->Pro substitution in mouse
      E-cadherin results in a complete gain of function. A correlation between cell
      permissivity and the nature of residue 16 in E-cadherins from several species is 
      established. The location of this key specificity residue in a region of
      E-cadherin not involved in cell-cell adhesion and the stringency of the
      interaction demonstrated here have important consequences not only for the
      understanding of internalin function but also for the choice of the animal model 
      to be used to study human listeriosis: mouse, albeit previously widely used, and 
      rat appear as inappropriate animal models to study all aspects of human
      listeriosis, as opposed to guinea-pig, which now stands as a small animal of
      choice for future in vivo studies.
FAU - Lecuit, M
AU  - Lecuit M
AD  - Unite des Interactions Bacteries-Cellules, Institut Pasteur, 75724 Paris Cedex
      15, France.
FAU - Dramsi, S
AU  - Dramsi S
FAU - Gottardi, C
AU  - Gottardi C
FAU - Fedor-Chaiken, M
AU  - Fedor-Chaiken M
FAU - Gumbiner, B
AU  - Gumbiner B
FAU - Cossart, P
AU  - Cossart P
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - EMBO J
JT  - The EMBO journal
JID - 8208664
RN  - 0 (Bacterial Proteins)
RN  - 0 (Cadherins)
RN  - 0 (Recombinant Fusion Proteins)
RN  - 139948-71-3 (internalin protein, Bacteria)
RN  - 9DLQ4CIU6V (Proline)
SB  - IM
SB  - S
MH  - Amino Acid Sequence
MH  - Amino Acid Substitution
MH  - Animals
MH  - Bacterial Proteins/*metabolism
MH  - Cadherins/*chemistry/*metabolism
MH  - Cell Line
MH  - Disease Models, Animal
MH  - Endocytosis
MH  - Female
MH  - Guinea Pigs
MH  - Humans
MH  - Listeria monocytogenes/*growth & development/*pathogenicity
MH  - Listeriosis/microbiology
MH  - Mice
MH  - Mice, Inbred BALB C
MH  - Models, Molecular
MH  - Molecular Sequence Data
MH  - Phylogeny
MH  - Proline/genetics/*metabolism
MH  - Protein Binding
MH  - Rats
MH  - Recombinant Fusion Proteins/chemistry/genetics/metabolism
MH  - Species Specificity
PMC - PMC1171471
EDAT- 1999/07/16 00:00
MHDA- 1999/07/16 00:01
CRDT- 1999/07/16 00:00
PHST- 1999/07/16 00:00 [pubmed]
PHST- 1999/07/16 00:01 [medline]
PHST- 1999/07/16 00:00 [entrez]
AID - 10.1093/emboj/18.14.3956 [doi]
PST - ppublish
SO  - EMBO J. 1999 Jul 15;18(14):3956-63. doi: 10.1093/emboj/18.14.3956.