PMID- 10405764
OWN - NLM
STAT- MEDLINE
DCOM- 19990927
LR  - 20190921
IS  - 0898-6568 (Print)
IS  - 0898-6568 (Linking)
VI  - 11
IP  - 7
DP  - 1999 Jul
TI  - Isolation, expression and analysis of splice variants of a human
      Ca2+/calmodulin-stimulated phosphodiesterase (PDE1A).
PG  - 535-44
AB  - The PDE1A gene encodes a Ca2+/calmodulin-stimulated 3',5'-cyclic nucleotide
      phosphodiesterase (PDE). We have performed 5' and 3' RACE and identified two
      additional 5'-splice variants and one additional 3'-splice variant of the human
      PDE1A gene. The three known 5'-splice variants and the two known 3'-splice
      variants combine to generate six different PDE1A mRNAs. However, one of the
      5'-splice variants exhibits alternate splicing in the 5' untranslated region.
      Thus the six mRNAs encode four different PDE1A proteins. Recombinant forms of the
      different human PDE1A isoforms were expressed in Sf9 cells. The kinetic
      properties and inhibitor sensitivities of the four PDE1A isoforms are very
      similar to one another.
FAU - Snyder, P B
AU  - Snyder PB
AD  - ICOS Corporation, Bothell, WA 98021, USA. psnyder@icos.com
FAU - Florio, V A
AU  - Florio VA
FAU - Ferguson, K
AU  - Ferguson K
FAU - Loughney, K
AU  - Loughney K
LA  - eng
SI  - GENBANK/AF110235
SI  - GENBANK/AF110236
SI  - GENBANK/AF110237
SI  - GENBANK/AF110238
SI  - GENBANK/AF110239
SI  - GENBANK/AF110240
PT  - Journal Article
PL  - England
TA  - Cell Signal
JT  - Cellular signalling
JID - 8904683
RN  - 0 (3' Untranslated Regions)
RN  - 0 (5' Untranslated Regions)
RN  - 0 (DNA, Complementary)
RN  - 0 (Isoenzymes)
RN  - EC 3.1.4.- (Phosphoric Diester Hydrolases)
RN  - EC 3.1.4.17 (3',5'-Cyclic-AMP Phosphodiesterases)
RN  - EC 3.1.4.17 (Cyclic Nucleotide Phosphodiesterases, Type 1)
RN  - EC 3.1.4.17 (PDE1A protein, human)
RN  - EC 3.1.4.35 (3',5'-Cyclic-GMP Phosphodiesterases)
SB  - IM
MH  - 3' Untranslated Regions
MH  - 3',5'-Cyclic-AMP Phosphodiesterases/*genetics/isolation & purification/metabolism
MH  - 3',5'-Cyclic-GMP Phosphodiesterases/*genetics/isolation & purification/metabolism
MH  - 5' Untranslated Regions
MH  - *Alternative Splicing
MH  - Animals
MH  - Base Sequence
MH  - Cell Line
MH  - Cyclic Nucleotide Phosphodiesterases, Type 1
MH  - DNA, Complementary
MH  - Gene Expression
MH  - Genetic Variation
MH  - Humans
MH  - Isoenzymes/genetics/isolation & purification/metabolism
MH  - Kinetics
MH  - Molecular Sequence Data
MH  - *Phosphoric Diester Hydrolases
MH  - Spodoptera/cytology
EDAT- 1999/07/16 00:00
MHDA- 1999/07/16 00:01
CRDT- 1999/07/16 00:00
PHST- 1999/07/16 00:00 [pubmed]
PHST- 1999/07/16 00:01 [medline]
PHST- 1999/07/16 00:00 [entrez]
AID - S0898-6568(99)00027-3 [pii]
AID - 10.1016/s0898-6568(99)00027-3 [doi]
PST - ppublish
SO  - Cell Signal. 1999 Jul;11(7):535-44. doi: 10.1016/s0898-6568(99)00027-3.