PMID- 10405341
OWN - NLM
STAT- MEDLINE
DCOM- 19990809
LR  - 20041117
IS  - 0006-291X (Print)
IS  - 0006-291X (Linking)
VI  - 261
IP  - 1
DP  - 1999 Jul 22
TI  - Gene expression of a novel cytochrome P450 of the CYP4F subfamily in human
      seminal vesicles.
PG  - 169-74
AB  - 19R-Hydroxyprostaglandins are major components of human seminal fluid. They are
      apparently formed in the seminal vesicles by NADPH-dependent
      omega2-hydroxylation. The hydroxylase is likely a cytochrome P450 (CYP), which
      has not been identified. To address this issue we studied gene expression of CYPs
      in human seminal vesicles (n = 4) with reverse-transcription polymerase chain
      reaction (RT-PCR). CYP1B1, CYP2E1, CYP2J2, CYP3A5, CYP4B1, and CYP4B1 with
      insertion of three nucleotides (Ser207) were detected in all subjects. RT-PCR
      with degenerate primers for the CYP4 family yielded a novel cDNA sequence, which 
      was derived from a previously reported genomic sequence on chromosome 19p13.1 and
      present in all subjects. cDNA cloning showed that the deduced amino acid sequence
      consisted of 520 amino acids. Northern blot analysis demonstrated mRNA
      transcripts of approximately 2.1 and approximately 2.3 kb. The deduced protein
      showed 81.2 and 76.7% amino acid identity with the human enzymes CYP4F2 and
      CYP4F3. The novel CYP was designated CYP4F8.
CI  - Copyright 1999 Academic Press.
FAU - Bylund, J
AU  - Bylund J
AD  - Department of Pharmaceutical Biosciences, Uppsala University, Sweden.
      Johan.Bylund@farmbio.uu.se
FAU - Finnstrom, N
AU  - Finnstrom N
FAU - Oliw, E H
AU  - Oliw EH
LA  - eng
SI  - GENBANK/AF133298
PT  - Journal Article
PL  - United States
TA  - Biochem Biophys Res Commun
JT  - Biochemical and biophysical research communications
JID - 0372516
RN  - 0 (DNA Primers)
RN  - 0 (Isoenzymes)
RN  - 0 (RNA, Messenger)
RN  - 9035-51-2 (Cytochrome P-450 Enzyme System)
RN  - EC 1.- (Mixed Function Oxygenases)
RN  - EC 1.14.14.1 (Aryl Hydrocarbon Hydroxylases)
RN  - EC 1.14.14.1 (CYP4F8 protein, human)
RN  - EC 1.14.14.1 (prostaglandin omega hydroxylases)
SB  - IM
MH  - Amino Acid Sequence
MH  - *Aryl Hydrocarbon Hydroxylases
MH  - Base Sequence
MH  - Blotting, Northern
MH  - Cloning, Molecular
MH  - Conserved Sequence/genetics
MH  - Cytochrome P-450 Enzyme System/chemistry/*genetics/metabolism
MH  - DNA Primers/genetics
MH  - Exons/genetics
MH  - *Gene Expression
MH  - Humans
MH  - Introns/genetics
MH  - Isoenzymes/chemistry/genetics/metabolism
MH  - Male
MH  - Mixed Function Oxygenases/chemistry/*genetics/metabolism
MH  - Molecular Sequence Data
MH  - RNA, Messenger/analysis/genetics/metabolism
MH  - Reverse Transcriptase Polymerase Chain Reaction
MH  - Seminal Vesicles/*enzymology/metabolism
MH  - Sequence Alignment
EDAT- 1999/07/16 00:00
MHDA- 1999/07/16 00:01
CRDT- 1999/07/16 00:00
PHST- 1999/07/16 00:00 [pubmed]
PHST- 1999/07/16 00:01 [medline]
PHST- 1999/07/16 00:00 [entrez]
AID - 10.1006/bbrc.1999.1011 [doi]
AID - S0006-291X(99)91011-2 [pii]
PST - ppublish
SO  - Biochem Biophys Res Commun. 1999 Jul 22;261(1):169-74. doi:
      10.1006/bbrc.1999.1011.