PMID- 10405317
OWN - NLM
STAT- MEDLINE
DCOM- 19990809
LR  - 20061115
IS  - 0006-291X (Print)
IS  - 0006-291X (Linking)
VI  - 261
IP  - 1
DP  - 1999 Jul 22
TI  - Cloning, expression, and chromosomal mapping of a human ganglioside sialidase.
PG  - 21-7
AB  - Here we report the cDNA sequence of a human ganglioside sialidase. The cDNA was
      isolated from a human brain cDNA library by screening with a 240 bp probe
      generated by polymerase chain reaction using primers based on the sequences of
      rat cytosolic and bovine membrane sialidases which we previously cloned. The 3.0 
      kb cDNA encodes an open reading frame of 436 amino acids containing a putative
      transmenbrane domain and an Arg-Ile-Pro and three Asp-box sequences
      characteristic of sialidases and showing overall 83% and 39% identities to the
      bovine and rat enzymes, respectively. Northern blot analysis revealed high
      expression in skeletal muscle and testis, but low level in kidney, placenta,
      lung, and digestive organs. Transient expression of the cDNA in COS-1 cells
      resulted in a 130-fold increase in sialidase activity compared to the control
      level, and the activity was found to be almost specific for gangliosides.
      Fluorescent in situ hybridization allowed the human sialidase gene localized to
      chromosome 11 at q 13.5.
CI  - Copyright 1999 Academic Press.
FAU - Wada, T
AU  - Wada T
AD  - Research Institute, Miyagi Prefectural Cancer Center, Natori, Miyagi, 981-1293,
      Japan.
FAU - Yoshikawa, Y
AU  - Yoshikawa Y
FAU - Tokuyama, S
AU  - Tokuyama S
FAU - Kuwabara, M
AU  - Kuwabara M
FAU - Akita, H
AU  - Akita H
FAU - Miyagi, T
AU  - Miyagi T
LA  - eng
SI  - GENBANK/AB008185
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Biochem Biophys Res Commun
JT  - Biochemical and biophysical research communications
JID - 0372516
RN  - EC 3.2.1.18 (GM3 ganglioside sialidase)
RN  - EC 3.2.1.18 (Neuraminidase)
SB  - IM
MH  - Amino Acid Sequence
MH  - Animals
MH  - Base Sequence
MH  - Blotting, Northern
MH  - COS Cells
MH  - Cattle
MH  - Cell Membrane/enzymology
MH  - Chromosomes, Human, Pair 11/genetics
MH  - Cloning, Molecular
MH  - *Gene Expression
MH  - Humans
MH  - Hydrogen-Ion Concentration
MH  - Molecular Sequence Data
MH  - Neuraminidase/*genetics/metabolism
MH  - *Physical Chromosome Mapping
MH  - Rats
MH  - Sequence Homology, Amino Acid
MH  - Substrate Specificity
MH  - Transfection
EDAT- 1999/07/16 00:00
MHDA- 1999/07/16 00:01
CRDT- 1999/07/16 00:00
PHST- 1999/07/16 00:00 [pubmed]
PHST- 1999/07/16 00:01 [medline]
PHST- 1999/07/16 00:00 [entrez]
AID - 10.1006/bbrc.1999.0973 [doi]
AID - S0006-291X(99)90973-7 [pii]
PST - ppublish
SO  - Biochem Biophys Res Commun. 1999 Jul 22;261(1):21-7. doi: 10.1006/bbrc.1999.0973.