PMID- 10405182
OWN - NLM
STAT- MEDLINE
DCOM- 19990805
LR  - 20190621
IS  - 0014-5793 (Print)
IS  - 0014-5793 (Linking)
VI  - 453
IP  - 3
DP  - 1999 Jun 25
TI  - Mutational analysis of sites in the translational regulator, PHAS-I, that are
      selectively phosphorylated by mTOR.
PG  - 387-90
AB  - Results obtained with PHAS-I proteins having Ser to Ala mutations in the five
      known phosphorylation sites indicate that mTOR preferentially phosphorylates
      Thr36 and Thr45. The effects of phosphorylating these sites on eIF4E binding were
      assessed in a far-Western analysis with a labeled eIF4E probe. Phosphorylation of
      Thr36 only slightly attenuated binding of PHAS-I to eIF4E, while phosphorylation 
      of Thr45 markedly inhibited binding. Phosphorylation of neither site affected the
      electrophoretic mobility of the protein, indicating that results of studies that 
      rely solely on a gel-shift assay to assess changes in PHAS-I phosphorylation must
      be interpreted with caution.
FAU - Yang, D
AU  - Yang D
AD  - Department of Pharmacology, University of Virginia School of Medicine,
      Charlottesville 22908, USA.
FAU - Brunn, G J
AU  - Brunn GJ
FAU - Lawrence, J C Jr
AU  - Lawrence JC Jr
LA  - eng
PT  - Journal Article
PL  - England
TA  - FEBS Lett
JT  - FEBS letters
JID - 0155157
RN  - 0 (Carrier Proteins)
RN  - 0 (Eukaryotic Initiation Factor-4E)
RN  - 0 (Peptide Initiation Factors)
RN  - 0 (Phosphoproteins)
RN  - 2ZD004190S (Threonine)
RN  - EC 2.7.- (Protein Kinases)
RN  - EC 2.7.1.- (Phosphotransferases (Alcohol Group Acceptor))
RN  - EC 2.7.1.1 (TOR Serine-Threonine Kinases)
RN  - EC 2.7.11.17 (Calcium-Calmodulin-Dependent Protein Kinases)
SB  - IM
MH  - Calcium-Calmodulin-Dependent Protein Kinases/metabolism
MH  - *Carrier Proteins
MH  - DNA Mutational Analysis
MH  - Eukaryotic Initiation Factor-4E
MH  - Peptide Initiation Factors/genetics/*metabolism
MH  - Phosphoproteins/genetics/*metabolism
MH  - Phosphorylation
MH  - Phosphotransferases (Alcohol Group Acceptor)/*metabolism
MH  - Protein Binding
MH  - *Protein Kinases
MH  - Substrate Specificity
MH  - TOR Serine-Threonine Kinases
MH  - Threonine/metabolism
EDAT- 1999/07/15 10:00
MHDA- 2001/03/28 10:01
CRDT- 1999/07/15 10:00
PHST- 1999/07/15 10:00 [pubmed]
PHST- 2001/03/28 10:01 [medline]
PHST- 1999/07/15 10:00 [entrez]
AID - S0014-5793(99)00762-0 [pii]
AID - 10.1016/s0014-5793(99)00762-0 [doi]
PST - ppublish
SO  - FEBS Lett. 1999 Jun 25;453(3):387-90. doi: 10.1016/s0014-5793(99)00762-0.