PMID- 10404641
OWN - NLM
STAT- MEDLINE
DCOM- 19990805
LR  - 20190921
IS  - 1357-2725 (Print)
IS  - 1357-2725 (Linking)
VI  - 31
IP  - 6
DP  - 1999 Jun
TI  - Molecular cloning and characterization of a cDNA encoding the human leucocyte
      vacuolar protein sorting (h1Vps45).
PG  - 683-94
AB  - We have isolated a novel cDNA clone from human leucocyte cDNA library, encoding a
      Sec1p-like vacuolar protein sorting (h1Vps45) which is believed to be implicated 
      in vesicular transportation. Although the deduced amino acid (AA) sequence of
      this cDNA has revealed 97% identity to other known mammalian vacuolar protein
      sorting, there is an extensive variation in nucleotide sequence in comparison to 
      that of three previously reported human (hVps45), rat (rVps45) and mouse (mVps45)
      vacuolar protein sorting (Vps45) cDNAs [1-3]. At the nucleotide sequence level
      h1Vps45 demonstrated 90% homology to the hVps45 and rVps45 and 89% identity to
      mVps45 with no significant homology in their noncoding regions. The 2.4 Kb mRNA
      corresponding to the h1Vps45 clone is widely distributed in a variety of human
      tissues expressing highest levels in peripheral blood mononuclear cells (PBMC),
      neutrophils, heart, spleen, and testis. The chromosomal mapping studies have
      demonstrated that the h1Vps45 is localized to long arm of human chromosome 1 at
      q21-q22. Our data indicates that we have isolated, characterized and mapped a
      novel cDNA encoding h1Vps45, which may play an important role in protein
      trafficking as well as have clinical significance in the release of inflammatory 
      mediators e.g. histamine, bradykinin and cytokine release.
FAU - Rajasekariah, P
AU  - Rajasekariah P
AD  - Department of Immunology, Repatriation General Hospital, Concord, University of
      Sydney, NSW, Australia. pooli@crgmail.crg.nsw.gov.au
FAU - Eyre, H J
AU  - Eyre HJ
FAU - Stanley, K K
AU  - Stanley KK
FAU - Walls, R S
AU  - Walls RS
FAU - Sutherland, G R
AU  - Sutherland GR
LA  - eng
SI  - GENBANK/AJ133421
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Netherlands
TA  - Int J Biochem Cell Biol
JT  - The international journal of biochemistry & cell biology
JID - 9508482
RN  - 0 (Carrier Proteins)
RN  - 0 (DNA, Complementary)
RN  - 0 (Membrane Proteins)
RN  - 0 (RNA, Messenger)
RN  - 0 (VPS45 protein, human)
RN  - 0 (Vesicular Transport Proteins)
RN  - 0 (Vps45 protein, mouse)
RN  - 0 (Vps45 protein, rat)
SB  - IM
MH  - Amino Acid Sequence
MH  - Base Sequence
MH  - Biological Transport
MH  - Carrier Proteins/chemistry/*genetics
MH  - Chromosome Mapping
MH  - Chromosomes, Human, Pair 1
MH  - Cloning, Molecular
MH  - DNA, Complementary/chemistry
MH  - Golgi Apparatus/metabolism
MH  - Humans
MH  - Leukocytes/*chemistry
MH  - Membrane Proteins/chemistry/*genetics
MH  - Molecular Sequence Data
MH  - RNA, Messenger/metabolism
MH  - Reverse Transcriptase Polymerase Chain Reaction
MH  - Sequence Alignment
MH  - *Vesicular Transport Proteins
EDAT- 1999/07/15 00:00
MHDA- 1999/07/15 00:01
CRDT- 1999/07/15 00:00
PHST- 1999/07/15 00:00 [pubmed]
PHST- 1999/07/15 00:01 [medline]
PHST- 1999/07/15 00:00 [entrez]
AID - S1357272599000175 [pii]
AID - 10.1016/s1357-2725(99)00017-5 [doi]
PST - ppublish
SO  - Int J Biochem Cell Biol. 1999 Jun;31(6):683-94. doi:
      10.1016/s1357-2725(99)00017-5.