PMID- 10404385
OWN - NLM
STAT- MEDLINE
DCOM- 19990830
LR  - 20061115
IS  - 0730-2312 (Print)
IS  - 0730-2312 (Linking)
VI  - 74
IP  - 2
DP  - 1999 Aug 1
TI  - Reassembling proteins and chaperones in human nuclear matrix protein fractions.
PG  - 145-51
AB  - To detect putative filament forming components, nuclear matrix proteins were
      searched for proteins extensively reassembling from urea solution. Eight
      proteins, ubiquitously occurring in various human cell types, but not apparent in
      the cytosol, were registered by means of two-dimensional gel electrophoresis.
      They consisted of a protein exhibiting a novel amino acid sequence; of nuclear
      lamin B2, RbAp46, and RbAp48; and of four as yet unknown proteins. Furthermore,
      partial sequencing, mass spectrometry, and immunodetection of proteins
      demonstrated the presence of molecular chaperones and protein folding catalysts
      in the nuclear matrix fractions. In addition to a TCP-1-related protein, certain 
      members of the heat shock, PDI, and calreticulin family of proteins were
      detected. On the basis of the absence of several other heat shock proteins in the
      nuclear matrix fraction, a general contamination by cytoplasmic chaperones
      appears unlikely.
FAU - Gerner, C
AU  - Gerner C
AD  - Institute of Cancer Research, University of Vienna, Austria.
      christopher.gerner@univie.ac.at
FAU - Holzmann, K
AU  - Holzmann K
FAU - Meissner, M
AU  - Meissner M
FAU - Gotzmann, J
AU  - Gotzmann J
FAU - Grimm, R
AU  - Grimm R
FAU - Sauermann, G
AU  - Sauermann G
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - J Cell Biochem
JT  - Journal of cellular biochemistry
JID - 8205768
RN  - 0 (Antigens, Nuclear)
RN  - 0 (Molecular Chaperones)
RN  - 0 (Nuclear Proteins)
SB  - IM
MH  - Amino Acid Sequence
MH  - Antigens, Nuclear
MH  - Electrophoresis, Gel, Two-Dimensional
MH  - Humans
MH  - Molecular Chaperones/chemistry/*metabolism
MH  - Nuclear Proteins/*metabolism
MH  - Protein Processing, Post-Translational
EDAT- 1999/07/15 10:00
MHDA- 2000/06/20 09:00
CRDT- 1999/07/15 10:00
PHST- 1999/07/15 10:00 [pubmed]
PHST- 2000/06/20 09:00 [medline]
PHST- 1999/07/15 10:00 [entrez]
AID - 10.1002/(SICI)1097-4644(19990801)74:2<145::AID-JCB1>3.0.CO;2-# [pii]
PST - ppublish
SO  - J Cell Biochem. 1999 Aug 1;74(2):145-51.