PMID- 10404304
OWN - NLM
STAT- MEDLINE
DCOM- 19990924
LR  - 20190921
IS  - 0919-8172 (Print)
IS  - 0919-8172 (Linking)
VI  - 6
IP  - 6
DP  - 1999 Jun
TI  - Expression of transitional cell-specific genes, uroplakin Ia and II, in bladder
      cancer: detection of circulating cancer cells in the peripheral blood of
      metastatic patients.
PG  - 286-92
AB  - BACKGROUND: Uroplakins (UP), urothelium-specific transmembrane proteins, are
      present only in urothelia and may be good candidates as tumor markers specific
      for transitional cell carcinomas (TCC). We investigated the expression of UP-Ia
      and UP-II genes in the tissues and peripheral blood of patients with TCC.
      METHODS: We investigated UP-Ia and UP-II gene expression in tissues from 12
      patients with TCC by reverse transcription-polymerase chain reaction (RT-PCR).
      HT1197, a TCC cell line, was used as an indicated cell line to assess a detection
      system for the UP-II gene-expressing cancer cells by nested RT-PCR. We also
      investigated UP-II gene expression in the peripheral blood of 12 other patients
      with TCC by nested RT-PCR. RESULTS: Prior to the investigation of UP-Ia and UP-II
      gene expression, a partial nucleotide sequence of human UP-II gene cDNA was
      determined to prepare the primers for RT-PCR. Uroplakin genes were expressed in
      both cancerous and non-cancerous urothelia taken from all patients examined by
      RT-PCR. The detection sensitivity of our assay showed that one cancer cell could 
      be detected in 5 mL peripheral blood. UP-II gene-expression was detected in the
      peripheral blood from all three patients with metastatic TCC but not from the
      nine patients with non-metastatic TCC nor the three healthy volunteers.
      CONCLUSIONS: Uroplakins may be employed as tumor markers for transitional cell
      cancer, because they are highly conserved and well expressed, not only in
      non-cancerous cells but also in cancer cells. Furthermore, detection of UP-II
      gene expression in blood by nested RT-PCR may provide helpful information in the 
      diagnosis and management of TCC.
FAU - Yuasa, T
AU  - Yuasa T
AD  - Department of Urology, Shiga University of Medical Science, Otsu, Japan.
      yoshiki@belle.shiga-med.ac.jp
FAU - Yoshiki, T
AU  - Yoshiki T
FAU - Isono, T
AU  - Isono T
FAU - Tanaka, T
AU  - Tanaka T
FAU - Hayashida, H
AU  - Hayashida H
FAU - Okada, Y
AU  - Okada Y
LA  - eng
SI  - GENBANK/AB009454
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Australia
TA  - Int J Urol
JT  - International journal of urology : official journal of the Japanese Urological
      Association
JID - 9440237
RN  - 0 (Biomarkers, Tumor)
RN  - 0 (DNA, Complementary)
RN  - 0 (Membrane Glycoproteins)
RN  - 0 (Membrane Proteins)
RN  - 0 (UPK1A protein, human)
RN  - 0 (UPK2 protein, human)
RN  - 0 (Uroplakin II)
RN  - 0 (Uroplakin Ia)
SB  - IM
MH  - Adult
MH  - Aged
MH  - Amino Acid Sequence
MH  - Base Sequence
MH  - Biomarkers, Tumor/blood
MH  - Carcinoma, Transitional Cell/genetics/*metabolism/pathology
MH  - DNA, Complementary/genetics
MH  - Female
MH  - Humans
MH  - Male
MH  - Membrane Glycoproteins/*biosynthesis/genetics
MH  - Membrane Proteins/*biosynthesis/blood/genetics
MH  - Middle Aged
MH  - Molecular Sequence Data
MH  - Neoplastic Cells, Circulating/*metabolism/pathology
MH  - Predictive Value of Tests
MH  - Reverse Transcriptase Polymerase Chain Reaction
MH  - Sensitivity and Specificity
MH  - Tumor Cells, Cultured
MH  - Urinary Bladder Neoplasms/genetics/*metabolism/pathology
MH  - Uroplakin II
MH  - Uroplakin Ia
EDAT- 1999/07/15 00:00
MHDA- 1999/07/15 00:01
CRDT- 1999/07/15 00:00
PHST- 1999/07/15 00:00 [pubmed]
PHST- 1999/07/15 00:01 [medline]
PHST- 1999/07/15 00:00 [entrez]
AID - 10.1046/j.1442-2042.1999.00064.x [doi]
PST - ppublish
SO  - Int J Urol. 1999 Jun;6(6):286-92. doi: 10.1046/j.1442-2042.1999.00064.x.