PMID- 10403824
OWN - NLM
STAT- MEDLINE
DCOM- 19990816
LR  - 20051117
IS  - 0006-291X (Print)
IS  - 0006-291X (Linking)
VI  - 260
IP  - 3
DP  - 1999 Jul 14
TI  - CAPN 8: isolation of a new mouse calpain-isoenzyme.
PG  - 671-5
AB  - Recent molecular biological approaches indicate that calpain, also named CANP for
      calcium-activated neutral protease and originally characterized as an
      intracellular cytoplasmatic nonlysosomal cysteine protease that requires calcium 
      ions for activity, constitutes a large superfamily consisting of ubiquitous and
      tissue specific homologues, which are widely distributed in cells of various
      organisms from human to fungus. Due to the increasing number of substrates along 
      with the involvement of calpain isoenzymes in mammalian diseases, especially in
      malignancies, members of the calpain superfamily seem to be important
      biomodulators in physiological as well as pathological cell function. Here we
      report the characterisation of a new calpain, named CAPN 8 with a different
      C-terminal domain, implicating a putative new regulatory mechanism. Northern blot
      analysis revealed an ubiquitous expression with different RNA levels in all
      tissues examined. Highest levels were found in brain, kidney, and digestive
      tract, suggesting a specific regulatory function of CAPN 8 in these tissues.
CI  - Copyright 1999 Academic Press.
FAU - Braun, C
AU  - Braun C
AD  - Institute of Human Genetics, University of Saar, Homburg/Saar, D-66421, Germany.
FAU - Engel, M
AU  - Engel M
FAU - Theisinger, B
AU  - Theisinger B
FAU - Welter, C
AU  - Welter C
FAU - Seifert, M
AU  - Seifert M
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Biochem Biophys Res Commun
JT  - Biochemical and biophysical research communications
JID - 0372516
RN  - 0 (Isoenzymes)
RN  - 0 (RNA, Messenger)
RN  - EC 3.4.22.- (Calpain)
RN  - EC 3.4.22.- (Capn8 protein, mouse)
SB  - IM
MH  - Amino Acid Sequence
MH  - Animals
MH  - Blotting, Northern
MH  - Brain/metabolism
MH  - Calpain/chemistry/*genetics/isolation & purification
MH  - Conserved Sequence/genetics
MH  - Contig Mapping
MH  - Digestive System/metabolism
MH  - Expressed Sequence Tags
MH  - Isoenzymes/chemistry/*genetics/isolation & purification
MH  - Kidney/metabolism
MH  - Mice
MH  - Molecular Sequence Data
MH  - Open Reading Frames/genetics
MH  - RNA, Messenger/genetics/metabolism
MH  - Sequence Alignment
EDAT- 1999/07/15 00:00
MHDA- 1999/07/15 00:01
CRDT- 1999/07/15 00:00
PHST- 1999/07/15 00:00 [pubmed]
PHST- 1999/07/15 00:01 [medline]
PHST- 1999/07/15 00:00 [entrez]
AID - 10.1006/bbrc.1999.0948 [doi]
AID - S0006-291X(99)90948-8 [pii]
PST - ppublish
SO  - Biochem Biophys Res Commun. 1999 Jul 14;260(3):671-5. doi:
      10.1006/bbrc.1999.0948.