PMID- 10403772
OWN - NLM
STAT- MEDLINE
DCOM- 19990805
LR  - 20061115
IS  - 0006-291X (Print)
IS  - 0006-291X (Linking)
VI  - 260
IP  - 2
DP  - 1999 Jul 5
TI  - Structure of mouse calpastatin isoforms: implications of species-common and
      species-specific alternative splicing.
PG  - 339-45
AB  - Mouse calpastatin cDNAs were cloned by the method of RT-PCR using RNA isolated
      from myoblast C2C12 cells. Nucleotide sequencing of the isolated clones revealed 
      an in-frame ATG codon upstream of the previously assigned translation initiation 
      methionine. Except for the N-terminal segment, the new translatable region
      (domain XL) was similar to the sequence of bovine calpastatin in which domain XL 
      was first identified. Among the isolated mouse calpastatin cDNA clones, three
      isoforms (mCS-a, mCS-b, and mCS-c) were identified. In domain L, mCS-b had a
      deletion of the region corresponding to exon 3 of the human calpastatin gene.
      RT-PCR analyses of various mouse tissues revealed that mCS-b was the major form
      and that the content of mCS-a, nondeleted form, was 5-10% in tissues including
      skeletal muscle, liver, brain, etc. and about 30% in the myoblast C2C12 cells.
      Unlike human and rat cDNAs, no other deletions were detected in mouse calpastatin
      domain L. Isolation of the cDNA clone of mCS-c, which lacked regions
      corresponding to exons 3 and 12, was obtained by chance because its expression
      level was under the detectable level in the mouse tissues and even in C2C12
      cells.
CI  - Copyright 1999 Academic Press.
FAU - Takano, J
AU  - Takano J
AD  - Department of Applied Molecular Biosciences, Graduate School of Bioagricultural
      Sciences, Nagoya University, Furo-cho, Chikusa-ku, Nagoya, 464-8601, Japan.
FAU - Kawamura, T
AU  - Kawamura T
FAU - Murase, M
AU  - Murase M
FAU - Hitomi, K
AU  - Hitomi K
FAU - Maki, M
AU  - Maki M
LA  - eng
SI  - GENBANK/AB026997
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Biochem Biophys Res Commun
JT  - Biochemical and biophysical research communications
JID - 0372516
RN  - 0 (Calcium-Binding Proteins)
RN  - 0 (DNA Primers)
RN  - 0 (Protein Isoforms)
RN  - 0 (RNA, Messenger)
RN  - 79079-11-1 (calpastatin)
SB  - IM
MH  - *Alternative Splicing
MH  - Amino Acid Sequence
MH  - Animals
MH  - Base Sequence
MH  - Calcium-Binding Proteins/*chemistry/genetics
MH  - Cell Line
MH  - Conserved Sequence
MH  - DNA Primers
MH  - Humans
MH  - Mice
MH  - Molecular Sequence Data
MH  - Protein Conformation
MH  - Protein Isoforms/*chemistry/genetics
MH  - RNA, Messenger/genetics
MH  - Sequence Deletion
MH  - Sequence Homology, Amino Acid
MH  - Species Specificity
EDAT- 1999/07/15 00:00
MHDA- 1999/07/15 00:01
CRDT- 1999/07/15 00:00
PHST- 1999/07/15 00:00 [pubmed]
PHST- 1999/07/15 00:01 [medline]
PHST- 1999/07/15 00:00 [entrez]
AID - 10.1006/bbrc.1999.0903 [doi]
AID - S0006-291X(99)90903-8 [pii]
PST - ppublish
SO  - Biochem Biophys Res Commun. 1999 Jul 5;260(2):339-45. doi:
      10.1006/bbrc.1999.0903.