PMID- 10403699 OWN - NLM STAT- MEDLINE DCOM- 19990917 LR - 20201209 IS - 0920-8569 (Print) IS - 0920-8569 (Linking) VI - 18 IP - 2 DP - 1999 TI - Complete nucleotide sequence of bean pod mottle virus RNA1: sequence comparisons and evolutionary relationships to other comoviruses. PG - 129-37 AB - The complete nucleotide sequence of bean pod mottle comovirus (BPMV) RNA 1 was determined. It is 5983 nucleotides long, excluding the poly(A) tail, and encodes a polyprotein of 1850 amino acid (aa) residues. Multiple alignments of the deduced aa sequence of BPMV polyprotein with those of cowpea mosaic virus (CPMV), red clover mottle virus (RCMV) and cowpea severe mosaic virus (CPSMV) indicated that BPMV RNA1 encodes the predicted set of five mature proteins: the equivalent of CPMV 32K protease cofactor, 58K putative helicase, VPg, 24K protease and 87K putative RNA-dependent RNA polymerase. Of the four proposed cleavage sites in BPMV RNA1 polyprotein, the one at the 32K/58K site (Q/A) is distinct for BPMV polyprotein and those at the 58K/VPg and VPg/24K junctions (Q/S and Q/M, respectively) are identical in all four comovirus polyproteins. Sequence comparison and phylogenetic analysis revealed that BPMV RNA1 is more closely related to CPSMV than to CPMV or to RCMV. FAU - Di, R AU - Di R AD - Department of Plant Pathology, University of Kentucky, Lexington 40546, USA. FAU - Hu, C C AU - Hu CC FAU - Ghabrial, S A AU - Ghabrial SA LA - eng SI - GENBANK/U70866 PT - Comparative Study PT - Journal Article PT - Research Support, Non-U.S. Gov't PL - United States TA - Virus Genes JT - Virus genes JID - 8803967 RN - 0 (RNA, Viral) RN - 0 (Viral Core Proteins) RN - 0 (Viral Proteins) RN - 0 (polyprotein, comovirus) RN - EC 2.7.7.48 (RNA-Dependent RNA Polymerase) RN - EC 3.6.4.13 (RNA Helicases) SB - IM MH - Amino Acid Sequence MH - Base Sequence MH - Comovirus/classification/*genetics MH - *Evolution, Molecular MH - Fabaceae/virology MH - Molecular Sequence Data MH - Phylogeny MH - Plants, Medicinal MH - RNA Helicases/genetics MH - RNA, Viral/*analysis MH - RNA-Dependent RNA Polymerase/genetics MH - Sequence Analysis, RNA MH - Sequence Homology, Amino Acid MH - Viral Core Proteins/genetics MH - Viral Proteins/genetics EDAT- 1999/07/14 00:00 MHDA- 1999/07/14 00:01 CRDT- 1999/07/14 00:00 PHST- 1999/07/14 00:00 [pubmed] PHST- 1999/07/14 00:01 [medline] PHST- 1999/07/14 00:00 [entrez] AID - 10.1023/a:1008012502470 [doi] PST - ppublish SO - Virus Genes. 1999;18(2):129-37. doi: 10.1023/a:1008012502470.