PMID- 10403563
OWN - NLM
STAT- MEDLINE
DCOM- 19990819
LR  - 20190720
IS  - 0304-3835 (Print)
IS  - 0304-3835 (Linking)
VI  - 140
IP  - 1-2
DP  - 1999 Jun 1
TI  - Identification of a novel gene, LDOC1, down-regulated in cancer cell lines.
PG  - 227-34
AB  - By screening for differentially expressed genes in cancer cells, using the RNA
      differential display (DD) technique, we identified a novel cDNA, LDOC1, that is
      down-regulated in some cancer cell lines. A Northern blot analysis revealed no
      expression in pancreatic and gastric cancer cell lines but ubiquitous expression 
      in normal human tissues. This new gene was mapped on chromosome Xq27 and the
      predicted protein sequence showed no similarity to known sequences in the
      database except for a leucine zipper-like motif at the N-terminal region and a
      proline-rich region that shares marked similarity to an SH3-binding domain. In an
      enhanced green fluorescent protein (EGFP) assay, the EGFP-LDOC1 fusion protein
      was localized in the nucleus. Although the function of LDOC1 is still unknown,
      our results suggest that this novel gene codes for a nuclear protein, and
      down-regulation of LDOC1 may have an important role in the development and/or
      progression of some cancers.
FAU - Nagasaki, K
AU  - Nagasaki K
AD  - Growth Factor Division, National Cancer Center Research Institute, Tokyo, Japan. 
      knagasak@gan2.ncc.go.jp
FAU - Manabe, T
AU  - Manabe T
FAU - Hanzawa, H
AU  - Hanzawa H
FAU - Maass, N
AU  - Maass N
FAU - Tsukada, T
AU  - Tsukada T
FAU - Yamaguchi, K
AU  - Yamaguchi K
LA  - eng
SI  - GENBANK/AB019527
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Ireland
TA  - Cancer Lett
JT  - Cancer letters
JID - 7600053
RN  - 0 (DNA, Complementary)
RN  - 0 (LDOC1 protein, human)
RN  - 0 (Nuclear Proteins)
RN  - 0 (Recombinant Fusion Proteins)
RN  - 0 (Tumor Suppressor Proteins)
RN  - 9DLQ4CIU6V (Proline)
SB  - IM
MH  - Amino Acid Sequence
MH  - Base Sequence
MH  - Blotting, Northern
MH  - Cell Nucleus/metabolism
MH  - Chromosome Mapping
MH  - Cloning, Molecular
MH  - DNA, Complementary/genetics/isolation & purification
MH  - Down-Regulation
MH  - Gene Expression
MH  - *Genes, Tumor Suppressor
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - Leucine Zippers/genetics
MH  - Molecular Sequence Data
MH  - Neoplasms, Experimental/*genetics
MH  - Nuclear Proteins/*genetics
MH  - Organ Specificity
MH  - Polymerase Chain Reaction
MH  - Proline/genetics
MH  - Recombinant Fusion Proteins/metabolism
MH  - Tumor Cells, Cultured
MH  - *Tumor Suppressor Proteins
MH  - X Chromosome/genetics
MH  - src Homology Domains/genetics
EDAT- 1999/07/14 00:00
MHDA- 1999/07/14 00:01
CRDT- 1999/07/14 00:00
PHST- 1999/07/14 00:00 [pubmed]
PHST- 1999/07/14 00:01 [medline]
PHST- 1999/07/14 00:00 [entrez]
AID - S0304-3835(99)00087-7 [pii]
AID - 10.1016/s0304-3835(99)00087-7 [doi]
PST - ppublish
SO  - Cancer Lett. 1999 Jun 1;140(1-2):227-34. doi: 10.1016/s0304-3835(99)00087-7.