PMID- 10403562
OWN - NLM
STAT- MEDLINE
DCOM- 19990819
LR  - 20190720
IS  - 0304-3835 (Print)
IS  - 0304-3835 (Linking)
VI  - 140
IP  - 1-2
DP  - 1999 Jun 1
TI  - Identification of a novel gene, DAM1, amplified at chromosome 1p13.3-21 region in
      human breast cancer cell lines.
PG  - 219-26
AB  - Screening for differentially expressed genes in cancer cell lines by the RNA
      differential display (DD) technique identified a novel mRNA that encodes a 26-kDa
      protein and is up-regulated in MCF-7 and BT-20 breast cancer cells. The predicted
      amino acid sequence showed 38% homology to Caenorhabditis elegans T12A2.7
      protein, indicating that this is a possible human homologue for C. elegans
      T12A2.7 protein. The mRNA, designated DAM1 (DNA amplified in mammary carcinoma), 
      was mapped at the 1p13.3-21 region, which is frequently altered in human breast
      cancer. By Southern blot analysis, we confirmed that the up-regulation of this
      novel gene is associated with gene amplification in MCF-7 (10-fold) and BT-20
      (5-fold) cells. Our findings suggest that the DAM1 gene is a novel gene
      up-regulated by amplification in human breast cancer cell lines.
FAU - Nagasaki, K
AU  - Nagasaki K
AD  - Growth Factor Division, National Cancer Center Research Institute, Tokyo, Japan. 
      knagasak@gan2.ncc.go.jp
FAU - Maass, N
AU  - Maass N
FAU - Manabe, T
AU  - Manabe T
FAU - Hanzawa, H
AU  - Hanzawa H
FAU - Tsukada, T
AU  - Tsukada T
FAU - Kikuchi, K
AU  - Kikuchi K
FAU - Yamaguchi, K
AU  - Yamaguchi K
LA  - eng
SI  - GENBANK/AB020623
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Ireland
TA  - Cancer Lett
JT  - Cancer letters
JID - 7600053
RN  - 0 (BCAS2 protein, human)
RN  - 0 (Neoplasm Proteins)
SB  - IM
MH  - Amino Acid Sequence
MH  - Animals
MH  - Base Sequence
MH  - Blotting, Southern
MH  - Breast Neoplasms/*genetics
MH  - Caenorhabditis elegans/genetics
MH  - Carcinoma/*genetics
MH  - Chromosome Mapping
MH  - Chromosomes, Human, Pair 1/genetics
MH  - Cloning, Molecular
MH  - Female
MH  - *Genes, Neoplasm
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - Molecular Sequence Data
MH  - Neoplasm Proteins/*genetics
MH  - Polymerase Chain Reaction
MH  - Sequence Homology, Amino Acid
MH  - Tissue Distribution
MH  - Tumor Cells, Cultured
EDAT- 1999/07/14 00:00
MHDA- 1999/07/14 00:01
CRDT- 1999/07/14 00:00
PHST- 1999/07/14 00:00 [pubmed]
PHST- 1999/07/14 00:01 [medline]
PHST- 1999/07/14 00:00 [entrez]
AID - S0304-3835(99)00091-9 [pii]
AID - 10.1016/s0304-3835(99)00091-9 [doi]
PST - ppublish
SO  - Cancer Lett. 1999 Jun 1;140(1-2):219-26. doi: 10.1016/s0304-3835(99)00091-9.