PMID- 10403379
OWN - NLM
STAT- MEDLINE
DCOM- 19990802
LR  - 20190621
IS  - 0014-5793 (Print)
IS  - 0014-5793 (Linking)
VI  - 453
IP  - 1-2
DP  - 1999 Jun 18
TI  - Ca2(+)-dependent interaction of N-copine, a member of the two C2 domain protein
      family, with OS-9, the product of a gene frequently amplified in osteosarcoma.
PG  - 77-80
AB  - N-copine is a novel two C2 domain protein that shows Ca2(+)-dependent
      phospholipid binding and membrane association. By using yeast two-hybrid assays, 
      we identified OS-9 as a protein capable of interacting with N-copine. We further 
      revealed that the second C2 domain of N-copine bound with the carboxy-terminal
      region of OS-9. Their interaction in vivo was also confirmed by
      co-immunoprecipitation from 293E cells co-expressing transfected N-copine and
      OS-9. In vitro binding assays showed that this interaction was Ca2(+)-dependent. 
      By Northern blot analysis, N-copine and OS-9 were co-expressed in the same
      regions of human brain. These results reveal that OS-9 is a potential target of
      N-copine.
FAU - Nakayama, T
AU  - Nakayama T
AD  - Department of Bacteriology, Kinki University School of Medicine, Osaka-Sayama,
      Osaka, Japan. nakayama@med.kindai.ac.jp
FAU - Yaoi, T
AU  - Yaoi T
FAU - Kuwajima, G
AU  - Kuwajima G
FAU - Yoshie, O
AU  - Yoshie O
FAU - Sakata, T
AU  - Sakata T
LA  - eng
PT  - Journal Article
PL  - England
TA  - FEBS Lett
JT  - FEBS letters
JID - 0155157
RN  - 0 (Carrier Proteins)
RN  - 0 (Lectins)
RN  - 0 (Neoplasm Proteins)
RN  - 0 (OS9 protein, human)
RN  - 0 (copine)
RN  - SY7Q814VUP (Calcium)
SB  - IM
MH  - Binding Sites
MH  - Brain Chemistry
MH  - Calcium/*metabolism
MH  - Carrier Proteins/genetics/isolation & purification/*metabolism
MH  - Cloning, Molecular
MH  - Gene Amplification
MH  - Humans
MH  - Lectins
MH  - Neoplasm Proteins/genetics/isolation & purification/*metabolism
MH  - Osteosarcoma
MH  - Protein Binding
MH  - Saccharomyces cerevisiae/genetics
MH  - Tissue Distribution
EDAT- 1999/07/14 00:00
MHDA- 1999/07/14 00:01
CRDT- 1999/07/14 00:00
PHST- 1999/07/14 00:00 [pubmed]
PHST- 1999/07/14 00:01 [medline]
PHST- 1999/07/14 00:00 [entrez]
AID - S0014-5793(99)00700-0 [pii]
AID - 10.1016/s0014-5793(99)00700-0 [doi]
PST - ppublish
SO  - FEBS Lett. 1999 Jun 18;453(1-2):77-80. doi: 10.1016/s0014-5793(99)00700-0.