PMID- 10403367
OWN - NLM
STAT- MEDLINE
DCOM- 19990802
LR  - 20190621
IS  - 0014-5793 (Print)
IS  - 0014-5793 (Linking)
VI  - 453
IP  - 1-2
DP  - 1999 Jun 18
TI  - The small GTPases Rab5a, Rab5b and Rab5c are differentially phosphorylated in
      vitro.
PG  - 20-4
AB  - Rab GTPases play a fundamental role in the regulation of membrane traffic. Three 
      different Rab5 isoforms have been reported but no differences in their function
      in endocytosis have been discovered. As the Rab5 isoforms show a conserved
      consensus site for Ser/Thr phosphorylation, we investigated whether this site was
      phosphorylated. Here, we report that the three Rab5 proteins are differentially
      recognized by different kinases. Rab5a is efficiently phosphorylated by
      extracellular-regulated kinase 1 but not by extracellular-regulated kinase 2,
      while cdc2 kinase preferentially phosphorylates Ser-123 of Rab5b. These findings 
      strongly suggest that phosphorylation could be important to differentially
      regulate the function of the Rab5 isoforms.
FAU - Chiariello, M
AU  - Chiariello M
AD  - Dipartimento di Biologia e Patologia Cellulare e Molecolare L. Califano and
      Centro di Endocrinologia ed Oncologia Sperimentale G. Salvatore del Consiglio
      Nazionale delle Ricerche, Napoli, Italy.
FAU - Bruni, C B
AU  - Bruni CB
FAU - Bucci, C
AU  - Bucci C
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - FEBS Lett
JT  - FEBS letters
JID - 0155157
RN  - 0 (Isoenzymes)
RN  - EC 2.7.11.17 (Calcium-Calmodulin-Dependent Protein Kinases)
RN  - EC 2.7.11.24 (JNK Mitogen-Activated Protein Kinases)
RN  - EC 2.7.11.24 (Mitogen-Activated Protein Kinase 3)
RN  - EC 2.7.11.24 (Mitogen-Activated Protein Kinases)
RN  - EC 3.6.1.- (GTP Phosphohydrolases)
RN  - EC 3.6.1.- (GTP-Binding Proteins)
RN  - EC 3.6.5.2 (rab5 GTP-Binding Proteins)
SB  - IM
MH  - Calcium-Calmodulin-Dependent Protein Kinases/*metabolism
MH  - GTP Phosphohydrolases/*metabolism
MH  - GTP-Binding Proteins/*metabolism
MH  - Isoenzymes/metabolism
MH  - JNK Mitogen-Activated Protein Kinases
MH  - Mitogen-Activated Protein Kinase 3
MH  - *Mitogen-Activated Protein Kinases
MH  - Phosphorylation
MH  - Substrate Specificity
MH  - rab5 GTP-Binding Proteins
EDAT- 1999/07/14 00:00
MHDA- 1999/07/14 00:01
CRDT- 1999/07/14 00:00
PHST- 1999/07/14 00:00 [pubmed]
PHST- 1999/07/14 00:01 [medline]
PHST- 1999/07/14 00:00 [entrez]
AID - S0014-5793(99)00686-9 [pii]
AID - 10.1016/s0014-5793(99)00686-9 [doi]
PST - ppublish
SO  - FEBS Lett. 1999 Jun 18;453(1-2):20-4. doi: 10.1016/s0014-5793(99)00686-9.