PMID- 10400745
OWN - NLM
STAT- MEDLINE
DCOM- 19990824
LR  - 20181113
IS  - 0022-538X (Print)
IS  - 0022-538X (Linking)
VI  - 73
IP  - 8
DP  - 1999 Aug
TI  - A putative cell surface receptor for anemia-inducing feline leukemia virus
      subgroup C is a member of a transporter superfamily.
PG  - 6500-5
AB  - Domestic cats infected with the horizontally transmitted feline leukemia virus
      subgroup A (FeLV-A) often produce mutants (termed FeLV-C) that bind to a distinct
      cell surface receptor and cause severe aplastic anemia in vivo and erythroblast
      destruction in bone marrow cultures. The major determinant for FeLV-C-induced
      anemia has been mapped to a small region of the surface envelope glycoprotein
      that is responsible for its receptor binding specificity. Thus, erythroblast
      destruction may directly or indirectly result from FeLV-C binding to its
      receptor. To address these issues, we functionally cloned a putative cell surface
      receptor for FeLV-C (FLVCR) by using a human T-lymphocyte cDNA library in a
      retroviral vector. Expression of the 2.0-kbp FLVCR cDNA in naturally resistant
      Swiss mouse fibroblasts and Chinese hamster ovary cells caused substantial
      susceptibility to FeLV-C but no change in susceptibilities to FeLV-B and other
      retroviruses. The predicted FLVCR protein contains 555 amino acids and 12
      hydrophobic potential membrane-spanning sequences. Database searches indicated
      that FLVCR is a member of the major-facilitator superfamily of transporters and
      implied that it may transport an organic anion. RNA blot analyses showed that
      FLVCR mRNA is expressed in multiple hematopoietic lineages rather than
      specifically in erythroblasts. These results suggest that the targeted
      destruction of erythroblasts by FeLV-C may derive from their greater sensitivity 
      to this virus rather than from a preferential susceptibility to infection.
FAU - Tailor, C S
AU  - Tailor CS
AD  - Department of Biochemistry and Molecular Biology, Oregon Health Sciences
      University, Portland, Oregon 97201-3098, USA. tailorc@ohsu.edu
FAU - Willett, B J
AU  - Willett BJ
FAU - Kabat, D
AU  - Kabat D
LA  - eng
SI  - GENBANK/AF118637
GR  - CA25810/CA/NCI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - J Virol
JT  - Journal of virology
JID - 0113724
RN  - 0 (Carrier Proteins)
RN  - 0 (DNA, Complementary)
RN  - 0 (Receptors, Virus)
RN  - 0 (feline leukemia virus receptor)
SB  - IM
MH  - 3T3 Cells
MH  - Amino Acid Sequence
MH  - Anemia, Aplastic/*virology
MH  - Animals
MH  - Base Sequence
MH  - CHO Cells
MH  - Caenorhabditis elegans
MH  - Carrier Proteins/chemistry/*classification/genetics
MH  - Cats
MH  - Cricetinae
MH  - DNA, Complementary
MH  - Gene Expression
MH  - Humans
MH  - Leukemia Virus, Feline/*metabolism
MH  - Mice
MH  - Molecular Sequence Data
MH  - Receptors, Virus/chemistry/*classification/genetics
MH  - Sequence Homology, Amino Acid
MH  - Tissue Distribution
PMC - PMC112732
EDAT- 1999/07/10 00:00
MHDA- 1999/07/10 00:01
CRDT- 1999/07/10 00:00
PHST- 1999/07/10 00:00 [pubmed]
PHST- 1999/07/10 00:01 [medline]
PHST- 1999/07/10 00:00 [entrez]
PST - ppublish
SO  - J Virol. 1999 Aug;73(8):6500-5.