PMID- 10400613
OWN - NLM
STAT- MEDLINE
DCOM- 19990819
LR  - 20190508
IS  - 0021-9258 (Print)
IS  - 0021-9258 (Linking)
VI  - 274
IP  - 29
DP  - 1999 Jul 16
TI  - CD39-L4 is a secreted human apyrase, specific for the hydrolysis of nucleoside
      diphosphates.
PG  - 20064-7
AB  - The human ecto-apyrase gene family consists of five reported members (CD39,
      CD39-L1, CD39-L2, CD39-L3, and CD39-L4). The family can be subdivided into two
      groups by conservation of proposed structural domains. The CD39, CD39-L1, and
      CD39-L3 genes all encode hydrophobic portions in their carboxy and amino termini,
      serving as transmembrane domains for CD39 and potentially for the other two
      members. CD39-L2 and CD39-L4 genes encode hydrophobic portions in their amino
      termini, suggesting that they might encode secreted apyrases. We demonstrate that
      the CD39-L4 gene encodes the first reported human secreted ecto-apyrase. COS-7
      cells transfected with a CD39-L4 expression construct utilizing the naturally
      occurring leader peptide express recombinant protein outside of the cells. This
      expression can be blocked by brefeldin A, a chemical that inhibits a step in
      mammalian secretory pathways. We also demonstrate expression of CD39-L4 message
      in macrophages, suggesting that the protein is present in the circulation.
      Furthermore, we show that CD39-L4 is an E-type apyrase, is dependent on calcium
      and magnesium cations, and has high degree of specificity for NDPs over NTPs as
      enzymatic substrates. A potential physiological role in hemostasis and platelet
      aggregation is presented.
FAU - Mulero, J J
AU  - Mulero JJ
AD  - Functional Genomics Department, Hyseq Inc., Sunnyvale, California 94086, USA.
FAU - Yeung, G
AU  - Yeung G
FAU - Nelken, S T
AU  - Nelken ST
FAU - Ford, J E
AU  - Ford JE
LA  - eng
PT  - Journal Article
PL  - United States
TA  - J Biol Chem
JT  - The Journal of biological chemistry
JID - 2985121R
RN  - 0 (Antigens, CD)
RN  - 0 (Cations, Divalent)
RN  - 0 (DNA Primers)
RN  - 61D2G4IYVH (Adenosine Diphosphate)
RN  - EC 3.6.1.- (Adenosine Triphosphatases)
RN  - EC 3.6.1.5 (Apyrase)
RN  - EC 3.6.1.5 (CD39 antigen)
SB  - IM
MH  - Adenosine Diphosphate/*metabolism
MH  - *Adenosine Triphosphatases
MH  - Animals
MH  - Antigens, CD/*metabolism
MH  - Apyrase/*metabolism
MH  - Base Sequence
MH  - COS Cells
MH  - Cations, Divalent
MH  - DNA Primers
MH  - Enzyme Activation
MH  - Humans
MH  - Hydrolysis
MH  - Substrate Specificity
EDAT- 1999/07/10 00:00
MHDA- 1999/07/10 00:01
CRDT- 1999/07/10 00:00
PHST- 1999/07/10 00:00 [pubmed]
PHST- 1999/07/10 00:01 [medline]
PHST- 1999/07/10 00:00 [entrez]
AID - 10.1074/jbc.274.29.20064 [doi]
PST - ppublish
SO  - J Biol Chem. 1999 Jul 16;274(29):20064-7. doi: 10.1074/jbc.274.29.20064.