PMID- 10400611
OWN - NLM
STAT- MEDLINE
DCOM- 19990819
LR  - 20210209
IS  - 0021-9258 (Print)
IS  - 0021-9258 (Linking)
VI  - 274
IP  - 29
DP  - 1999 Jul 16
TI  - Activation of the lck tyrosine-protein kinase by the binding of the tip protein
      of herpesvirus saimiri in the absence of regulatory tyrosine phosphorylation.
PG  - 20056-9
AB  - The Tip protein of herpesvirus saimiri 484 binds to the Lck tyrosine-protein
      kinase at two sites and activates it dramatically. Lck has been shown previously 
      to be activated by either phosphorylation of Tyr394 or dephosphorylation of
      Tyr505. We examined here whether a change in the phosphorylation of either site
      was required for the activation of Lck by Tip. Remarkably, mutation of both
      regulatory sites of tyrosine phosphorylation did not prevent activation of Lck by
      Tip either in vivo or in a cell free in vitro system. Tip therefore appears to be
      able to activate Lck through an induced conformational change that does not
      necessarily involve altered phosphorylation of the kinase. Tip may represent the 
      prototype of a novel type of regulator of tyrosine-protein kinases.
FAU - Hartley, D A
AU  - Hartley DA
AD  - Molecular Biology and Virology Laboratory, The Salk Institute, La Jolla,
      California 92037, USA.
FAU - Hurley, T R
AU  - Hurley TR
FAU - Hardwick, J S
AU  - Hardwick JS
FAU - Lund, T C
AU  - Lund TC
FAU - Medveczky, P G
AU  - Medveczky PG
FAU - Sefton, B M
AU  - Sefton BM
LA  - eng
GR  - CA14195/CA/NCI NIH HHS/United States
GR  - CA42350/CA/NCI NIH HHS/United States
GR  - CA42364/CA/NCI NIH HHS/United States
GR  - etc.
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - J Biol Chem
JT  - The Journal of biological chemistry
JID - 2985121R
RN  - 0 (Phosphoproteins)
RN  - 0 (Viral Proteins)
RN  - 0 (tyrosine kinase interacting protein, Saimiriine herpesvirus 2)
RN  - EC 2.7.10.2 (Lymphocyte Specific Protein Tyrosine Kinase p56(lck))
SB  - IM
MH  - Cell Line
MH  - Enzyme Activation
MH  - Herpesvirus 2, Saimiriine/*metabolism
MH  - Humans
MH  - Lymphocyte Specific Protein Tyrosine Kinase p56(lck)/*metabolism
MH  - Mutagenesis
MH  - Phosphoproteins/*metabolism
MH  - Phosphorylation
MH  - Protein Binding
MH  - Viral Proteins/*metabolism
EDAT- 1999/07/10 00:00
MHDA- 1999/07/10 00:01
CRDT- 1999/07/10 00:00
PHST- 1999/07/10 00:00 [pubmed]
PHST- 1999/07/10 00:01 [medline]
PHST- 1999/07/10 00:00 [entrez]
AID - 10.1074/jbc.274.29.20056 [doi]
AID - S0021-9258(19)72612-X [pii]
PST - ppublish
SO  - J Biol Chem. 1999 Jul 16;274(29):20056-9. doi: 10.1074/jbc.274.29.20056.