PMID- 10399937
OWN - NLM
STAT- MEDLINE
DCOM- 19991028
LR  - 20211108
IS  - 0896-6273 (Print)
IS  - 0896-6273 (Linking)
VI  - 23
IP  - 2
DP  - 1999 Jun
TI  - Synaptic clustering of AMPA receptors by the extracellular immediate-early gene
      product Narp.
PG  - 309-23
AB  - Narp (neuronal activity-regulated pentraxin) is a secreted immediate-early gene
      (IEG) regulated by synaptic activity in brain. In this study, we demonstrate that
      Narp possesses several properties that make it likely to play a key role in
      excitatory synaptogenesis. Narp is shown to be selectively enriched at excitatory
      synapses on neurons from both the hippocampus and spinal cord. Overexpression of 
      recombinant Narp increases the number of excitatory but not inhibitory synapses
      in cultured spinal neurons. In transfected HEK 293T cells, Narp interacts with
      itself, forming large surface clusters that coaggregate AMPA receptor subunits.
      Moreover, Narp-expressing HEK 293T cells can induce the aggregation of neuronal
      AMPA receptors. These studies support a model in which Narp functions as an
      extracellular aggregating factor for AMPA receptors.
FAU - O'Brien, R J
AU  - O'Brien RJ
AD  - Howard Hughes Medical Institute, and Department of Neuroscience, Johns Hopkins
      University School of Medicine, Baltimore, Maryland 21205, USA.
FAU - Xu, D
AU  - Xu D
FAU - Petralia, R S
AU  - Petralia RS
FAU - Steward, O
AU  - Steward O
FAU - Huganir, R L
AU  - Huganir RL
FAU - Worley, P
AU  - Worley P
LA  - eng
GR  - R01 NS036715/NS/NINDS NIH HHS/United States
GR  - K08NS01652/NS/NINDS NIH HHS/United States
GR  - R01NS36715/NS/NINDS NIH HHS/United States
GR  - R01MH53608/MH/NIMH NIH HHS/United States
PT  - Journal Article
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Neuron
JT  - Neuron
JID - 8809320
RN  - 0 (Immediate-Early Proteins)
RN  - 0 (Nerve Tissue Proteins)
RN  - 0 (Receptors, AMPA)
RN  - 0 (glutamate receptor ionotropic, AMPA 3)
RN  - 0 (neuronal pentraxin)
RN  - 9007-41-4 (C-Reactive Protein)
RN  - TFZ3H25BS1 (glutamate receptor ionotropic, AMPA 1)
SB  - IM
CIN - Neuron. 1999 Jun;23(2):195-7. PMID: 10399923
MH  - Animals
MH  - Axons/metabolism
MH  - Blotting, Western
MH  - C-Reactive Protein/biosynthesis/*physiology
MH  - Cell Line
MH  - Cells, Cultured
MH  - Dendrites/metabolism
MH  - Extracellular Space/metabolism/physiology
MH  - Hippocampus/cytology
MH  - Immediate-Early Proteins/biosynthesis/*physiology
MH  - Immunohistochemistry
MH  - Microscopy, Electron
MH  - Nerve Tissue Proteins/biosynthesis/*physiology
MH  - Neurons/cytology/metabolism
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Receptors, AMPA/biosynthesis/metabolism/*physiology
MH  - Spinal Cord/cytology
MH  - Synapses/metabolism/*physiology
MH  - Transfection
EDAT- 1999/07/10 00:00
MHDA- 1999/07/10 00:01
CRDT- 1999/07/10 00:00
PHST- 1999/07/10 00:00 [pubmed]
PHST- 1999/07/10 00:01 [medline]
PHST- 1999/07/10 00:00 [entrez]
AID - S0896-6273(00)80782-5 [pii]
AID - 10.1016/s0896-6273(00)80782-5 [doi]
PST - ppublish
SO  - Neuron. 1999 Jun;23(2):309-23. doi: 10.1016/s0896-6273(00)80782-5.