PMID- 10398046
OWN - NLM
STAT- MEDLINE
DCOM- 19990803
LR  - 20091119
IS  - 0021-9967 (Print)
IS  - 0021-9967 (Linking)
VI  - 410
IP  - 4
DP  - 1999 Aug 9
TI  - Expression and localisation of dynamin and syntaxin during neural development and
      neuromuscular synapse formation.
PG  - 531-40
AB  - The expression and subcellular localisation of dynamin and syntaxin were examined
      during the periods of motor neuron development and neuromuscular synaptogenesis
      in the mouse embryo. Both dynamin and syntaxin could be detected by
      immunoblotting in the spinal cord at embryonic day 10 (E10; 2 days before axon
      outgrowth) and at all subsequent ages examined. Reverse transcription and
      polymerase chain reaction (RT-PCR) identified low levels of all three
      carboxy-terminal splicing forms of dynamin I in spinal cord from as early as E10.
      During the period of maturation of spinal neurons, from E10 to the first
      postnatal day (P0), the short carboxy-terminal splicing form of dynamin I
      (dynamin I*b) was up-regulated, as was dynamin III, relative to dynamin II mRNA. 
      Syntaxin immunostaining became colocalized with the synaptic vesicle protein,
      SV2, at neuromuscular synapses within 12 hours of the commencement of synapse
      formation and throughout subsequent development. In contrast, dynamin, which is
      important for activity-dependent synaptic vesicle recycling and, thus, sustained 
      neurotransmission, could not be detected at most newly formed synapses until
      several days after synapse formation. The delayed appearance of dynamin at the
      synapse, thus, heralds the neonatal development of robust synaptic transmission
      at the neuromuscular junction.
CI  - Copyright 1999 Wiley-Liss, Inc.
FAU - Noakes, P G
AU  - Noakes PG
AD  - Department of Physiology & Pharmacology, University of Queensland, 4072 Brisbane,
      Australia.
FAU - Chin, D
AU  - Chin D
FAU - Kim, S S
AU  - Kim SS
FAU - Liang, S
AU  - Liang S
FAU - Phillips, W D
AU  - Phillips WD
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - J Comp Neurol
JT  - The Journal of comparative neurology
JID - 0406041
RN  - 0 (Membrane Proteins)
RN  - 0 (Protein Isoforms)
RN  - 0 (Qa-SNARE Proteins)
RN  - EC 3.5.1.50 (Dynamin I)
RN  - EC 3.6.1.- (GTP Phosphohydrolases)
RN  - EC 3.6.5.5 (Dynamin III)
RN  - EC 3.6.5.5 (Dynamins)
SB  - IM
MH  - Animals
MH  - Animals, Newborn/*metabolism
MH  - Dynamin I
MH  - Dynamin III
MH  - Dynamins
MH  - Embryo, Mammalian/metabolism/*physiology
MH  - Embryonic and Fetal Development/physiology
MH  - GTP Phosphohydrolases/*metabolism
MH  - Membrane Proteins/*metabolism
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - Nervous System/*embryology/metabolism
MH  - Neuromuscular Junction/*embryology/metabolism
MH  - Protein Isoforms/metabolism
MH  - Qa-SNARE Proteins
MH  - Spinal Cord/embryology
MH  - Synapses/*physiology
MH  - Tissue Distribution/physiology
EDAT- 1999/07/09 00:00
MHDA- 1999/07/09 00:01
CRDT- 1999/07/09 00:00
PHST- 1999/07/09 00:00 [pubmed]
PHST- 1999/07/09 00:01 [medline]
PHST- 1999/07/09 00:00 [entrez]
AID - 10.1002/(SICI)1096-9861(19990809)410:4<531::AID-CNE2>3.0.CO;2-C [pii]
PST - ppublish
SO  - J Comp Neurol. 1999 Aug 9;410(4):531-40.