PMID- 10397761
OWN - NLM
STAT- MEDLINE
DCOM- 19990827
LR  - 20181113
IS  - 1059-1524 (Print)
IS  - 1059-1524 (Linking)
VI  - 10
IP  - 7
DP  - 1999 Jul
TI  - Nuclear import of sterol regulatory element-binding protein-2, a basic
      helix-loop-helix-leucine zipper (bHLH-Zip)-containing transcription factor,
      occurs through the direct interaction of importin beta with HLH-Zip.
PG  - 2221-33
AB  - The sterol regulatory element-binding protein-2 (SREBP-2) is produced as a large 
      precursor molecule attached to the endoplasmic reticulum membrane. In response to
      the sterol depletion, the N-terminal segment of the precursor, which contains a
      basic helix-loop-helix-leucine zipper domain, is released by two sequential
      cleavages and is translocated to the nucleus, where it activates the
      transcription of target genes. The data herein show that released SREBP-2 uses a 
      distinct nuclear transport pathway, which is mediated by importin beta. The
      mature form of SREBP-2 is actively transported into the nucleus when injected
      into the cell cytoplasm. SREBP-2 binds directly to importin beta in the absence
      of importin alpha. Ran-GTP but not Ran-GDP causes the dissociation of the
      SREBP-2-importin beta complex. G19VRan-GTP inhibits the nuclear import of SREBP-2
      in living cells. In the permeabilized cell in vitro transport system, nuclear
      import of SREBP-2 is reconstituted only by importin beta in conjunction with Ran 
      and its interacting protein p10/NTF2. We further demonstrate that the
      helix-loop-helix-leucine zipper motif of SREBP-2 contains a novel type of nuclear
      localization signal, which binds directly to importin beta.
FAU - Nagoshi, E
AU  - Nagoshi E
AD  - Department of Anatomy and Cell Biology, Osaka University Medical School, Osaka
      565-0871, Japan.
FAU - Imamoto, N
AU  - Imamoto N
FAU - Sato, R
AU  - Sato R
FAU - Yoneda, Y
AU  - Yoneda Y
LA  - eng
GR  - U17 CE002006/CE/NCIPC CDC HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Mol Biol Cell
JT  - Molecular biology of the cell
JID - 9201390
RN  - 0 (DNA-Binding Proteins)
RN  - 0 (Karyopherins)
RN  - 0 (Nuclear Proteins)
RN  - 0 (Recombinant Proteins)
RN  - 0 (SREBF2 protein, human)
RN  - 0 (Sterol Regulatory Element Binding Protein 2)
RN  - 0 (Transcription Factors)
RN  - EC 3.6.5.2 (ran GTP-Binding Protein)
SB  - IM
MH  - Binding Sites
MH  - Biological Transport
MH  - Cell Nucleus/*metabolism
MH  - DNA-Binding Proteins/genetics/*metabolism
MH  - HeLa Cells/metabolism
MH  - Helix-Loop-Helix Motifs
MH  - Humans
MH  - Karyopherins
MH  - Leucine Zippers
MH  - Nuclear Proteins/*metabolism
MH  - Recombinant Proteins/genetics/metabolism
MH  - Sterol Regulatory Element Binding Protein 2
MH  - Transcription Factors/genetics/*metabolism
MH  - ran GTP-Binding Protein
PMC - PMC25438
EDAT- 1999/07/09 00:00
MHDA- 1999/07/09 00:01
CRDT- 1999/07/09 00:00
PHST- 1999/07/09 00:00 [pubmed]
PHST- 1999/07/09 00:01 [medline]
PHST- 1999/07/09 00:00 [entrez]
AID - 10.1091/mbc.10.7.2221 [doi]
PST - ppublish
SO  - Mol Biol Cell. 1999 Jul;10(7):2221-33. doi: 10.1091/mbc.10.7.2221.