PMID- 10397721
OWN - NLM
STAT- MEDLINE
DCOM- 19990805
LR  - 20141120
IS  - 0006-4971 (Print)
IS  - 0006-4971 (Linking)
VI  - 94
IP  - 2
DP  - 1999 Jul 15
TI  - The receptor protein tyrosine phosphatase, PTP-RO, is upregulated during
      megakaryocyte differentiation and Is associated with the c-Kit receptor.
PG  - 539-49
AB  - We have recently isolated a cDNA encoding a novel human receptor-type tyrosine
      phosphatase, termed PTP-RO (for a protein tyrosine phosphatase receptor omicron),
      from 5-fluorouracil-treated murine bone marrow cells. PTP-RO is a human homologue
      of murine PTPlambda and is related to the homotypically adhering kappa and mu
      receptor-type tyrosine phosphatases. PTP-RO is expressed in human megakaryocytic 
      cell lines, primary bone marrow megakaryocytes, and stem cells. PTP-RO mRNA and
      protein expression are upregulated upon phorbol 12-myristate 13-acetate (PMA)
      treatment of the megakaryocytic cell lines CMS, CMK, and Dami. To elucidate the
      function of PTP-RO in megakaryocytic cells and its potential involvement in the
      stem cell factor (SCF)/c-Kit receptor pathway, COS-7 and 293 cells were
      cotransfected with the cDNAs of both the c-Kit tyrosine kinase receptor and
      PTP-RO. PTP-RO was found to be associated with the c-Kit receptor in these
      transfected cells and the SCF/Kit ligand induced a rapid tyrosine phosphorylation
      of PTP-RO. Interestingly, these transfected cells demonstrated a decrease in
      their proliferative response to the SCF/Kit ligand. In addition, we assessed the 
      association of PTP-RO with c-Kit in vivo. The results demonstrated that PTP-RO
      associates with c-Kit but not with the tyrosine kinase receptor FGF-R and that
      PTP-RO is tyrosine-phosphorylated after SCF stimulation of Mo7e and CMK cells.
      Antisense oligonucleotides directed against PTP-RO mRNA sequences significantly
      inhibited megakaryocyte progenitor proliferation. Therefore, these data show that
      the novel tyrosine kinase phosphatase PTP-RO is involved in megakaryocytopoiesis 
      and that its function is mediated by the SCF/c-Kit pathway.
FAU - Taniguchi, Y
AU  - Taniguchi Y
AD  - Division of Experimental Medicine, Beth Israel Deaconess Medical Center, Harvard 
      Institutes of Medicine, Boston, MA, USA.
FAU - London, R
AU  - London R
FAU - Schinkmann, K
AU  - Schinkmann K
FAU - Jiang, S
AU  - Jiang S
FAU - Avraham, H
AU  - Avraham H
LA  - eng
GR  - HL 51456/HL/NHLBI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Blood
JT  - Blood
JID - 7603509
RN  - 0 (CTNNB1 protein, human)
RN  - 0 (CTNNB1 protein, mouse)
RN  - 0 (Cadherins)
RN  - 0 (Cytoskeletal Proteins)
RN  - 0 (Oligonucleotides, Antisense)
RN  - 0 (Recombinant Fusion Proteins)
RN  - 0 (Stem Cell Factor)
RN  - 0 (Trans-Activators)
RN  - 0 (beta Catenin)
RN  - EC 2.7.10.1 (Proto-Oncogene Proteins c-kit)
RN  - EC 3.1.3.48 (PTPRO protein, human)
RN  - EC 3.1.3.48 (PTPRU protein, human)
RN  - EC 3.1.3.48 (Protein Tyrosine Phosphatases)
RN  - EC 3.1.3.48 (Ptpro protein, mouse)
RN  - EC 3.1.3.48 (Ptpru protein, mouse)
RN  - EC 3.1.3.48 (Receptor-Like Protein Tyrosine Phosphatases, Class 2)
RN  - EC 3.1.3.48 (Receptor-Like Protein Tyrosine Phosphatases, Class 3)
RN  - NI40JAQ945 (Tetradecanoylphorbol Acetate)
SB  - AIM
SB  - IM
MH  - Adolescent
MH  - Animals
MH  - COS Cells
MH  - Cadherins/metabolism
MH  - Cell Differentiation/drug effects
MH  - Cell Line
MH  - Cercopithecus aethiops
MH  - Cytoskeletal Proteins/metabolism
MH  - Enzyme Induction/drug effects
MH  - Female
MH  - *Gene Expression Regulation, Developmental/drug effects
MH  - Hematopoiesis
MH  - Humans
MH  - Leukemia, Myeloid, Acute/pathology
MH  - Megakaryocytes/*cytology/enzymology
MH  - Mice
MH  - Oligonucleotides, Antisense/pharmacology
MH  - Phosphorylation
MH  - Protein Processing, Post-Translational
MH  - Protein Tyrosine Phosphatases/*biosynthesis/chemistry/genetics
MH  - Proto-Oncogene Proteins c-kit/genetics/*metabolism
MH  - Receptor-Like Protein Tyrosine Phosphatases, Class 2
MH  - Receptor-Like Protein Tyrosine Phosphatases, Class 3
MH  - Recombinant Fusion Proteins/metabolism
MH  - Stem Cell Factor/pharmacology
MH  - Tetradecanoylphorbol Acetate/pharmacology
MH  - *Trans-Activators
MH  - Transfection
MH  - Tumor Cells, Cultured
MH  - beta Catenin
EDAT- 1999/07/09 00:00
MHDA- 1999/07/09 00:01
CRDT- 1999/07/09 00:00
PHST- 1999/07/09 00:00 [pubmed]
PHST- 1999/07/09 00:01 [medline]
PHST- 1999/07/09 00:00 [entrez]
PST - ppublish
SO  - Blood. 1999 Jul 15;94(2):539-49.