PMID- 10397268
OWN - NLM
STAT- MEDLINE
DCOM- 19990728
LR  - 20190816
IS  - 0008-5472 (Print)
IS  - 0008-5472 (Linking)
VI  - 59
IP  - 13
DP  - 1999 Jul 1
TI  - Isolation of a novel gene, TSP50, by a hypomethylated DNA fragment in human
      breast cancer.
PG  - 3215-21
AB  - A novel gene, testes-specific protease 50 (TSP50), was isolated from a human
      testes cDNA library by using a genomic DNA probe, BR50. BR50 was isolated by a
      modified representational difference analysis (RDA) technique due to its
      hypomethylated feature in a breast cancer biopsy. This altered DNA methylation
      status was also detected by BR50 in other breast and some ovarian cancer tissues.
      The TSP50 gene product is a homologue to several human proteases, which indicates
      that it may encode a protease-like protein. Northern analysis of 16 different
      types of normal human tissues suggests that TSP50 was highly and specifically
      expressed in human testes, which indicates that it might possess a unique
      biological function(s) in that organ. Methylation status analysis in normal human
      testes and other tissues showed a correlation between DNA methylation and gene
      expression. Most importantly, reverse transcription-PCR analysis of 18 paired
      breast cancer tissues found that in 28% of the cancer samples, the TSP50 gene was
      differentially expressed. The possibility that TSP50 may be an oncogene is
      presently under investigation.
FAU - Yuan, L
AU  - Yuan L
AD  - Molecular Oncology, Hematology/Oncology Medicine, North Shore-Long Island Jewish 
      Health System, New York University School of Medicine, Manhasset 11030, USA.
FAU - Shan, J
AU  - Shan J
FAU - De Risi, D
AU  - De Risi D
FAU - Broome, J
AU  - Broome J
FAU - Lovecchio, J
AU  - Lovecchio J
FAU - Gal, D
AU  - Gal D
FAU - Vinciguerra, V
AU  - Vinciguerra V
FAU - Xu, H P
AU  - Xu HP
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, Non-P.H.S.
PL  - United States
TA  - Cancer Res
JT  - Cancer research
JID - 2984705R
RN  - 0 (DNA Probes)
RN  - 0 (DNA, Neoplasm)
RN  - EC 3.4.- (Cathepsins)
RN  - EC 3.4.22.- (Cysteine Endopeptidases)
RN  - EC 3.4.22.43 (CTSV protein, human)
SB  - IM
MH  - Amino Acid Sequence
MH  - Base Sequence
MH  - Blotting, Northern
MH  - Breast/enzymology
MH  - Breast Neoplasms/enzymology/*genetics
MH  - Cathepsins/*genetics
MH  - Chromosome Mapping
MH  - *Chromosomes, Human, Pair 3
MH  - Cysteine Endopeptidases/*genetics
MH  - DNA Methylation
MH  - DNA Probes
MH  - DNA, Neoplasm/genetics
MH  - Female
MH  - Gene Library
MH  - Humans
MH  - Male
MH  - Molecular Sequence Data
MH  - Ovarian Neoplasms/enzymology/*genetics
MH  - Ovary/enzymology
MH  - Polymerase Chain Reaction
MH  - Reference Values
MH  - Testis/enzymology
EDAT- 1999/07/09 00:00
MHDA- 1999/07/09 00:01
CRDT- 1999/07/09 00:00
PHST- 1999/07/09 00:00 [pubmed]
PHST- 1999/07/09 00:01 [medline]
PHST- 1999/07/09 00:00 [entrez]
PST - ppublish
SO  - Cancer Res. 1999 Jul 1;59(13):3215-21.