PMID- 10397266
OWN - NLM
STAT- MEDLINE
DCOM- 19990728
LR  - 20101118
IS  - 0008-5472 (Print)
IS  - 0008-5472 (Linking)
VI  - 59
IP  - 13
DP  - 1999 Jul 1
TI  - Testisin, a new human serine proteinase expressed by premeiotic testicular germ
      cells and lost in testicular germ cell tumors.
PG  - 3199-205
AB  - We have cloned and characterized a cDNA encoding a new human serine proteinase,
      testisin, that is abundantly expressed only in the testis and is lost in
      testicular tumors. The testisin cDNA was identified by homology cloning using
      degenerate primers directed at conserved sequence motifs within the catalytic
      regions of serine proteinases. It is 1073 nucleotides long, including 942
      nucleotides of open reading frame and a 113-nucleotide 3' untranslated sequence. 
      Northern and dot blot analyses of RNA from a range of normal human tissues
      revealed a 1.4-kb mRNA species that was present only in testis, which was not
      detected in eight of eight testicular tumors. Testisin cDNA is predicted to
      encode a protein of 314 amino acids, which consists of a 19-amino acid (aa)
      signal peptide, a 22-aa proregion, and a 273-aa catalytic domain, including a
      unique 17-aa COOH-terminal hydrophobic extension that is predicted to function as
      a membrane anchor. The deduced amino acid sequence of testisin shows 44% identity
      to prostasin and contains features that are typical of serine proteinases with
      trypsin-like substrate specificity. Antipeptide antibodies directed against the
      testisin polypeptide detected an immunoreactive testisin protein of Mr
      35,000-39,000 in cell lysates from COS-7 cells that were transiently transfected 
      with testisin cDNA. Immunostaining of normal testicular tissue showed that
      testisin was expressed in the cytoplasm and on the plasma membrane of premeiotic 
      germ cells. No staining was detected in eight of eight germ cell-derived
      testicular tumors. In addition, the testisin gene was localized by fluorescence
      in situ hybridization to the short arm of human chromosome 16 (16p13.3), a region
      that has been associated with allellic imbalance and loss of heterozygosity in
      sporadic testicular tumors. These findings demonstrate a new cell surface serine 
      proteinase, loss of which may have a direct or indirect role in the progression
      of testicular tumors of germ cell origin.
FAU - Hooper, J D
AU  - Hooper JD
AD  - Cellular Oncology Laboratory, University of Queensland Joint Oncology Program and
      Queensland Institute of Medical Research, Brisbane, Australia.
FAU - Nicol, D L
AU  - Nicol DL
FAU - Dickinson, J L
AU  - Dickinson JL
FAU - Eyre, H J
AU  - Eyre HJ
FAU - Scarman, A L
AU  - Scarman AL
FAU - Normyle, J F
AU  - Normyle JF
FAU - Stuttgen, M A
AU  - Stuttgen MA
FAU - Douglas, M L
AU  - Douglas ML
FAU - Loveland, K A
AU  - Loveland KA
FAU - Sutherland, G R
AU  - Sutherland GR
FAU - Antalis, T M
AU  - Antalis TM
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Cancer Res
JT  - Cancer research
JID - 2984705R
RN  - 0 (GPI-Linked Proteins)
RN  - 0 (Membrane Proteins)
RN  - 0 (RNA, Messenger)
RN  - 0 (Recombinant Proteins)
RN  - EC 3.4.21.- (PRSS21 protein, human)
RN  - EC 3.4.21.- (Serine Endopeptidases)
SB  - IM
MH  - Adult
MH  - Amino Acid Sequence
MH  - Base Sequence
MH  - Blotting, Northern
MH  - Catalytic Domain
MH  - Cloning, Molecular
MH  - GPI-Linked Proteins
MH  - Germinoma/*enzymology/genetics
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - Male
MH  - Membrane Proteins
MH  - Molecular Sequence Data
MH  - Molecular Weight
MH  - Polymerase Chain Reaction
MH  - RNA, Messenger/genetics
MH  - Recombinant Proteins/biosynthesis/chemistry
MH  - Reference Values
MH  - Sequence Alignment
MH  - Sequence Homology, Amino Acid
MH  - Serine Endopeptidases/biosynthesis/chemistry/*genetics
MH  - Spermatozoa/*enzymology
MH  - Testicular Neoplasms/*enzymology/genetics
MH  - Testis/enzymology
MH  - Transcription, Genetic
EDAT- 1999/07/09 00:00
MHDA- 1999/07/09 00:01
CRDT- 1999/07/09 00:00
PHST- 1999/07/09 00:00 [pubmed]
PHST- 1999/07/09 00:01 [medline]
PHST- 1999/07/09 00:00 [entrez]
PST - ppublish
SO  - Cancer Res. 1999 Jul 1;59(13):3199-205.