PMID- 10397259
OWN - NLM
STAT- MEDLINE
DCOM- 19990728
LR  - 20181201
IS  - 0008-5472 (Print)
IS  - 0008-5472 (Linking)
VI  - 59
IP  - 13
DP  - 1999 Jul 1
TI  - Characterization of the GAGE genes that are expressed in various human cancers
      and in normal testis.
PG  - 3157-65
AB  - The GAGE-1 gene was identified previously as a gene that codes for an antigenic
      peptide, YRPRPRRY, which was presented on a human melanoma by HLA-Cw6 molecules
      and recognized by a clone of CTLs derived from the patient bearing the tumor. By 
      screening a cDNA library from this melanoma, we identified five additional,
      closely related genes named GAGE-2-6. We report here that further screening of
      this library led to the identification of two more genes, GAGE-7B and -8. GAGE-1,
      -2, and -8 code for peptide YRPRPRRY. Using another antitumor CTL clone isolated 
      from the same melanoma patient, we identified antigenic peptide, YYWPRPRRY, which
      is encoded by GAGE-3, -4, -5, -6, and -7B and which is presented by HLA-A29
      molecules. Genomic cloning of GAGE-7B showed that it is composed of five exons.
      Sequence alignment showed that an additional exon, which is present only in the
      mRNA of GAGE-1, has been disrupted in gene GAGE-7B by the insertion of a long
      interspersed repeated element retroposon. These GAGE genes are located in the
      p11.2-p11.4 region of chromosome X. They are not expressed in normal tissues,
      except in testis, but a large proportion of tumors of various histological
      origins express at least one of these genes. Treatment of normal and tumor
      cultured cells with a demethylating agent, azadeoxycytidine, resulted in the
      transcriptional activation of GAGE genes, suggesting that their expression in
      tumors results from a demethylation process.
FAU - De Backer, O
AU  - De Backer O
AD  - Ludwig Institute for Cancer Research, Universite Catholique de Louvain, Brussels,
      Belgium. debacker@licr.ucl.ac.be
FAU - Arden, K C
AU  - Arden KC
FAU - Boretti, M
AU  - Boretti M
FAU - Vantomme, V
AU  - Vantomme V
FAU - De Smet, C
AU  - De Smet C
FAU - Czekay, S
AU  - Czekay S
FAU - Viars, C S
AU  - Viars CS
FAU - De Plaen, E
AU  - De Plaen E
FAU - Brasseur, F
AU  - Brasseur F
FAU - Chomez, P
AU  - Chomez P
FAU - Van den Eynde, B
AU  - Van den Eynde B
FAU - Boon, T
AU  - Boon T
FAU - van der Bruggen, P
AU  - van der Bruggen P
LA  - eng
SI  - GENBANK/AF055473
SI  - GENBANK/AF055474
SI  - GENBANK/AF055475
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Cancer Res
JT  - Cancer research
JID - 2984705R
RN  - 0 (Antigens, Neoplasm)
RN  - 0 (Neoplasm Proteins)
RN  - 0 (Recombinant Proteins)
RN  - 776B62CQ27 (Decitabine)
RN  - M801H13NRU (Azacitidine)
SB  - IM
MH  - Amino Acid Sequence
MH  - Animals
MH  - Antigens, Neoplasm/biosynthesis/chemistry/*genetics
MH  - Azacitidine/analogs & derivatives/pharmacology
MH  - Base Sequence
MH  - COS Cells
MH  - Chromosome Mapping
MH  - Cloning, Molecular
MH  - Decitabine
MH  - Exons
MH  - Gene Expression Regulation, Neoplastic/drug effects
MH  - Gene Library
MH  - Humans
MH  - Male
MH  - Melanoma/*genetics
MH  - Molecular Sequence Data
MH  - *Multigene Family
MH  - Neoplasm Proteins/biosynthesis/chemistry/*genetics
MH  - Neoplasms/*genetics
MH  - Recombinant Proteins/biosynthesis/chemistry
MH  - Sequence Alignment
MH  - Sequence Homology, Amino Acid
MH  - Sequence Homology, Nucleic Acid
MH  - Testis/*metabolism
MH  - Transcriptional Activation
MH  - Transfection
MH  - Tumor Cells, Cultured
MH  - X Chromosome
EDAT- 1999/07/09 00:00
MHDA- 1999/07/09 00:01
CRDT- 1999/07/09 00:00
PHST- 1999/07/09 00:00 [pubmed]
PHST- 1999/07/09 00:01 [medline]
PHST- 1999/07/09 00:00 [entrez]
PST - ppublish
SO  - Cancer Res. 1999 Jul 1;59(13):3157-65.