PMID- 10395998
OWN - NLM
STAT- MEDLINE
DCOM- 19990806
LR  - 20171101
IS  - 1660-8151 (Print)
IS  - 1660-8151 (Linking)
VI  - 82
IP  - 3
DP  - 1999
TI  - Expression and localization of the neuronal glycine receptor beta-subunit in
      human, rabbit and rat kidneys.
PG  - 254-60
AB  - The glycine receptor (GlyR) is a ligand-gated Cl- channel composed of two
      transmembrane subunits, alpha and beta, and gephyrin. The goal of this study was 
      to determine whether the alpha- and/or beta-subunits of the GlyR are expressed in
      human, rabbit and/or rat kidneys. Screening of human and rat kidney cortex cDNA
      libraries identified polymerase chain reaction products that were identical to
      the neuronal GlyR beta-subunit. Sequencing revealed that rat kidney cortex and
      neuronal GlyR beta-subunits were identical. RNA isolated from the S2 segment of
      rabbit renal proximal tubules (RPT) and rat and rabbit kidney cortex was
      amplified following reverse transcription and gave similar results to that of
      human and rat kidney cDNA libraries. Degenerate primers against all GlyR
      alpha-subunits did not yield a product from rat and rabbit kidney cortex RNA, or 
      from human and rat kidney cortex cDNA libraries. Immunofluorescence studies
      localized the beta-subunit and gephyrin to the basolateral membrane of rabbit
      RPT. These results provide compelling evidence for the GlyR beta-subunit, but not
      the alpha-subunit, in human, rabbit and rat kidney cortex.
FAU - Sarang, S S
AU  - Sarang SS
AD  - Division of Toxicology, University of Arkansas for Medical Sciences, Little Rock 
      72205-7199, USA.
FAU - Miller, G W
AU  - Miller GW
FAU - Grant, D F
AU  - Grant DF
FAU - Schnellmann, R G
AU  - Schnellmann RG
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Switzerland
TA  - Nephron
JT  - Nephron
JID - 0331777
RN  - 0 (Carrier Proteins)
RN  - 0 (GLRB protein, human)
RN  - 0 (Glrb protein, rat)
RN  - 0 (Membrane Proteins)
RN  - 0 (Receptors, Glycine)
RN  - 0 (gephyrin)
SB  - IM
MH  - Animals
MH  - Carrier Proteins/metabolism
MH  - Gene Expression Regulation/*genetics
MH  - Humans
MH  - Immunohistochemistry
MH  - Kidney Cortex/cytology/*metabolism
MH  - Membrane Proteins/metabolism
MH  - Rabbits
MH  - Rats
MH  - Receptors, Glycine/*genetics/metabolism
MH  - Restriction Mapping
MH  - Reverse Transcriptase Polymerase Chain Reaction
EDAT- 1999/07/09 00:00
MHDA- 1999/07/09 00:01
CRDT- 1999/07/09 00:00
PHST- 1999/07/09 00:00 [pubmed]
PHST- 1999/07/09 00:01 [medline]
PHST- 1999/07/09 00:00 [entrez]
AID - 45410 [pii]
AID - 10.1159/000045410 [doi]
PST - ppublish
SO  - Nephron. 1999;82(3):254-60. doi: 10.1159/000045410.