PMID- 10395968
OWN - NLM
STAT- MEDLINE
DCOM- 19990811
LR  - 20190610
IS  - 0006-3002 (Print)
IS  - 0006-3002 (Linking)
VI  - 1439
IP  - 1
DP  - 1999 Jul 9
TI  - Identification and characterization of the mouse cDNA encoding
      acyl-CoA:dihydroxyacetone phosphate acyltransferase.
PG  - 89-94
AB  - We used the amino acid sequence of human acyl-CoA:dihydroxyacetone phosphate
      acyltransferase (DHAPAT) as bait to screen the database of expressed sequence
      tags (dbEST) and identified several partial mouse cDNA clones showing high
      identity. Primers were selected based on the dbEST sequences and used for
      amplification of this transcript from cDNA prepared from mouse skin fibroblasts. 
      The complete nucleotide sequence was then determined and revealed an open reading
      frame (ORF) of 2034 bp encoding a protein consisting of 678 amino acids with a
      calculated molecular mass of 76870. The deduced amino acid sequence showed high
      identity (80%) with that of human DHAPAT and also revealed a typical peroxisomal 
      targeting signal type 1 (PTS1) at its extreme carboxy-terminus
      (alanine-lysine-leucine, AKL). Definitive evidence that this cDNA indeed codes
      for DHAPAT was obtained by heterologous expression in the yeast Saccharomyces
      cerevisiae. Northern blot analysis revealed high expression of DHAPAT especially 
      in mouse heart, liver and testis.
FAU - Ofman, R
AU  - Ofman R
AD  - Department of Clinical Chemistry and Pediatrics, University of Amsterdam,
      Academic Medical Centre, Meibergdreef 9, 1105 AZ, Amsterdam, The Netherlands.
FAU - Hogenhout, E M
AU  - Hogenhout EM
FAU - Wanders, R J
AU  - Wanders RJ
LA  - eng
SI  - GENBANK/AF110769
PT  - Journal Article
PL  - Netherlands
TA  - Biochim Biophys Acta
JT  - Biochimica et biophysica acta
JID - 0217513
RN  - 0 (DNA, Complementary)
RN  - EC 2.3.- (Acyltransferases)
RN  - EC 2.3.1.42 (glycerone-phosphate O-acyltransferase)
SB  - IM
MH  - Acyltransferases/*genetics
MH  - Amino Acid Sequence
MH  - Animals
MH  - Base Sequence
MH  - DNA, Complementary/*chemistry
MH  - Fibroblasts/enzymology
MH  - Gene Expression
MH  - Mice
MH  - Molecular Sequence Data
MH  - Polymerase Chain Reaction
MH  - Saccharomyces cerevisiae/genetics
MH  - Sequence Alignment
MH  - Sequence Tagged Sites
EDAT- 1999/07/09 00:00
MHDA- 1999/07/09 00:01
CRDT- 1999/07/09 00:00
PHST- 1999/07/09 00:00 [pubmed]
PHST- 1999/07/09 00:01 [medline]
PHST- 1999/07/09 00:00 [entrez]
AID - S1388-1981(99)00081-5 [pii]
AID - 10.1016/s1388-1981(99)00081-5 [doi]
PST - ppublish
SO  - Biochim Biophys Acta. 1999 Jul 9;1439(1):89-94. doi:
      10.1016/s1388-1981(99)00081-5.