PMID- 10395927
OWN - NLM
STAT- MEDLINE
DCOM- 19990819
LR  - 20190610
IS  - 0006-3002 (Print)
IS  - 0006-3002 (Linking)
VI  - 1446
IP  - 1-2
DP  - 1999 Jul 7
TI  - Interferon regulatory factor 1 tryptophan 11 to arginine point mutation abolishes
      DNA binding.
PG  - 140-4
AB  - Interferon regulatory factor-1 (IRF-1) is a transcriptional activator of genes
      induced by a variety of cytokines and growth factors. Defects in IRF-1 occur
      frequently in human cancers and may contribute to tumorigenesis. The IRF family
      of transcription factors share invariant tryptophan residues that have been
      proposed to function by orienting the DNA contacting residues of IRF-1 with the
      DNA core sequence of the IRF element. Here we describe a point mutation in IRF-1 
      that converts the tryptophan at codon 11 to arginine (W11R). The IRF-1 (W11R)
      mutation abolishes IRF-1 DNA binding and transactivating activities demonstrating
      the critical role of this invariant tryptophan in IRF-1 function.
FAU - Eason, D D
AU  - Eason DD
AD  - Department of Biochemistry and Molecular Biology, University of South Florida,
      College of Medicine, MDC 7, 12901 Bruce B. Downs Blvd., Tampa, FL 33612, USA.
FAU - Shepherd, A T
AU  - Shepherd AT
FAU - Blanck, G
AU  - Blanck G
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Netherlands
TA  - Biochim Biophys Acta
JT  - Biochimica et biophysica acta
JID - 0217513
RN  - 0 (DNA-Binding Proteins)
RN  - 0 (IRF1 protein, human)
RN  - 0 (Interferon Regulatory Factor-1)
RN  - 0 (Phosphoproteins)
RN  - 0 (Transcription Factors)
RN  - 8DUH1N11BX (Tryptophan)
RN  - 94ZLA3W45F (Arginine)
SB  - IM
MH  - Arginine/*chemistry
MH  - Carcinoma, Non-Small-Cell Lung
MH  - DNA-Binding Proteins/chemistry/*genetics/metabolism
MH  - Electrophoresis/methods
MH  - Humans
MH  - Interferon Regulatory Factor-1
MH  - Lung Neoplasms
MH  - Phosphoproteins/chemistry/*genetics/metabolism
MH  - Point Mutation
MH  - Reverse Transcriptase Polymerase Chain Reaction
MH  - Transcription Factors/chemistry/*genetics/metabolism
MH  - Transcription, Genetic
MH  - Transfection
MH  - Tryptophan/*chemistry
MH  - Tumor Cells, Cultured
MH  - Urinary Bladder Neoplasms
EDAT- 1999/07/09 00:00
MHDA- 1999/07/09 00:01
CRDT- 1999/07/09 00:00
PHST- 1999/07/09 00:00 [pubmed]
PHST- 1999/07/09 00:01 [medline]
PHST- 1999/07/09 00:00 [entrez]
AID - S0167-4781(99)00078-0 [pii]
AID - 10.1016/s0167-4781(99)00078-0 [doi]
PST - ppublish
SO  - Biochim Biophys Acta. 1999 Jul 7;1446(1-2):140-4. doi:
      10.1016/s0167-4781(99)00078-0.