PMID- 10395919
OWN - NLM
STAT- MEDLINE
DCOM- 19990819
LR  - 20190610
IS  - 0006-3002 (Print)
IS  - 0006-3002 (Linking)
VI  - 1446
IP  - 1-2
DP  - 1999 Jul 7
TI  - A novel subgroup of class I G-protein-coupled receptors.
PG  - 57-70
AB  - Based on structural similarities of an expressed sequence tag with the
      platelet-activating factor (PAF) receptor a cDNA clone encoding a novel
      G-protein-coupled receptor (GPCR), named GPR34, was isolated from a human fetal
      brain cDNA library. Genomic DNA analyses revealed the receptor to be encoded by
      an intronless single-copy gene at Xp11. 3-11.4. The predicted 381-amino-acid
      protein disclosed all structural features characteristic of a member of the class
      I GPCR family. Except an obvious sequence homology in transmembrane domain 6, no 
      further similarities to the PAF receptor or any other known GPCR were found. The 
      corresponding mouse receptor DNA was isolated from a genomic P1 library
      displaying a 90% amino acid identity compared to the human receptor. Phylogenetic
      studies showed that GPR34 is preserved among vertebrates, and the existence of
      GPR34 subtypes was demonstrated. The receptor mRNA is abundantly expressed in
      human and mouse tissues. In addition to the major 2-kb transcript, a 4-kb
      transcript was found only in mouse liver and testis. Expression of the human
      GPR34 in COS-7 cells followed by Western blot studies revealed specific bands of 
      a highly glycosylated protein between 75 and 90 kDa. A number of potential
      ligands including phospholipids, leukotrienes, hydroxy-eicosatetraenoic acids,
      nucleotides and peptides were tested in functional assays. Since none of the
      applied substances led to significant changes in second messenger levels (cAMP
      and inositol phosphates), the natural ligand and coupling profile of this novel
      GPCR subgroup remains unknown.
FAU - Schoneberg, T
AU  - Schoneberg T
AD  - Institut fur Pharmakologie, Universitatsklinikum Benjamin Franklin, Freie
      Universitat Berlin, Thielallee 69-73, D-14195, Berlin, Germany.
      schoberg@zedat.fu-berlin.de
FAU - Schulz, A
AU  - Schulz A
FAU - Grosse, R
AU  - Grosse R
FAU - Schade, R
AU  - Schade R
FAU - Henklein, P
AU  - Henklein P
FAU - Schultz, G
AU  - Schultz G
FAU - Gudermann, T
AU  - Gudermann T
LA  - eng
SI  - GENBANK/AF039686
SI  - GENBANK/AF081916
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Netherlands
TA  - Biochim Biophys Acta
JT  - Biochimica et biophysica acta
JID - 0217513
RN  - 0 (G-protein-coupled receptor 34)
RN  - 0 (Platelet Membrane Glycoproteins)
RN  - 0 (RNA, Messenger)
RN  - 0 (Receptors, Cell Surface)
RN  - 0 (Receptors, G-Protein-Coupled)
RN  - 0 (Receptors, Lysophospholipid)
RN  - 0 (Receptors, Purinergic P2)
RN  - 0 (platelet activating factor receptor)
RN  - EC 3.6.1.- (GTP-Binding Proteins)
SB  - IM
MH  - Amino Acid Sequence
MH  - Animals
MH  - Base Sequence
MH  - Brain/embryology/metabolism
MH  - COS Cells
MH  - Cloning, Molecular
MH  - GTP-Binding Proteins/*metabolism
MH  - Gene Expression Regulation
MH  - Gene Library
MH  - Humans
MH  - Mice
MH  - Molecular Sequence Data
MH  - Phylogeny
MH  - Platelet Membrane Glycoproteins/chemistry/genetics
MH  - RNA, Messenger/analysis
MH  - Receptors, Cell Surface/*classification/genetics/metabolism
MH  - *Receptors, G-Protein-Coupled
MH  - Receptors, Lysophospholipid
MH  - Receptors, Purinergic P2/chemistry/genetics
MH  - Sequence Alignment
EDAT- 1999/07/09 00:00
MHDA- 1999/07/09 00:01
CRDT- 1999/07/09 00:00
PHST- 1999/07/09 00:00 [pubmed]
PHST- 1999/07/09 00:01 [medline]
PHST- 1999/07/09 00:00 [entrez]
AID - S0167-4781(99)00081-0 [pii]
AID - 10.1016/s0167-4781(99)00081-0 [doi]
PST - ppublish
SO  - Biochim Biophys Acta. 1999 Jul 7;1446(1-2):57-70. doi:
      10.1016/s0167-4781(99)00081-0.