PMID- 10395895
OWN - NLM
STAT- MEDLINE
DCOM- 19990817
LR  - 20190707
IS  - 0378-1119 (Print)
IS  - 0378-1119 (Linking)
VI  - 234
IP  - 2
DP  - 1999 Jul 8
TI  - Identification of four novel ADAMs with potential roles in spermatogenesis and
      fertilization.
PG  - 227-37
AB  - The ADAM (A Disintegrin And Metalloprotease) family is known to have important
      roles in various developmental systems, e.g., myogenesis and neurogenesis. In
      this study, we searched for ADAMs that may function in spermatogenesis or
      fertilization, and have cloned and sequenced four new mouse ADAM cDNAs: ADAM 24, 
      ADAM 25, ADAM 26 and ADAM 27. The deduced amino acid sequences show that all four
      contain the complete domain organization common to ADAM family members. Messenger
      RNA for each of the four ADAMs was found only in the testis. The conserved
      zinc-dependent metalloprotease active site HEXGHXXGXXHD was found in the
      metalloprotease domain of three of the novel ADAMs, suggesting that they are
      testis-specific proteases, to which we give the alternative names: testase 1,
      ADAM 24; testase 2, ADAM 25; and testase 3, ADAM 26. Using RNA extracted from
      testes of pre-pubertal males of increasing age (8-40days), we found that adult
      levels of transcription, assessed in Northern blots, are reached by day 20 (ADAM 
      27), day 25 (ADAMs 24 and 25) and in the range day 25-50 (ADAM 26). These results
      suggest that each ADAM is transcribed in spermatogenic cells in a regulated
      pattern at a specific developmental stage.
FAU - Zhu, G Z
AU  - Zhu GZ
AD  - Department of Cell Biology and Human Anatomy, School of Medicine, University of
      California Davis, Davis, CA 95616, USA. gzhu@ucdavis.edu
FAU - Lin, Y
AU  - Lin Y
FAU - Myles, D G
AU  - Myles DG
FAU - Primakoff, P
AU  - Primakoff P
LA  - eng
SI  - GENBANK/AF167402
SI  - GENBANK/AF167403
SI  - GENBANK/AF167404
SI  - GENBANK/AF167405
SI  - GENBANK/AF167406
GR  - U54-29125/PHS HHS/United States
PT  - Journal Article
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - Netherlands
TA  - Gene
JT  - Gene
JID - 7706761
RN  - 0 (DNA, Complementary)
RN  - 0 (Disintegrins)
RN  - 0 (Membrane Glycoproteins)
RN  - 0 (RNA, Messenger)
RN  - EC 3.4.24.- (ADAM Proteins)
RN  - EC 3.4.24.- (Adam18 protein, mouse)
RN  - EC 3.4.24.- (Adam24 protein, mouse)
RN  - EC 3.4.24.- (Adam25 protein, mouse)
RN  - EC 3.4.24.- (Adam26a protein, mouse)
RN  - EC 3.4.24.- (Metalloendopeptidases)
SB  - IM
MH  - ADAM Proteins
MH  - Amino Acid Sequence
MH  - Animals
MH  - Base Sequence
MH  - Cloning, Molecular
MH  - DNA, Complementary/chemistry/genetics
MH  - Disintegrins/*genetics/physiology
MH  - Evolution, Molecular
MH  - Fertilization/*physiology
MH  - Gene Expression Regulation, Developmental
MH  - Male
MH  - Membrane Glycoproteins/*genetics
MH  - Metalloendopeptidases/*genetics/physiology
MH  - Mice
MH  - Molecular Sequence Data
MH  - Phylogeny
MH  - RNA, Messenger/genetics/metabolism
MH  - Sequence Alignment
MH  - Sequence Analysis, DNA
MH  - Sequence Homology, Amino Acid
MH  - Spermatogenesis/*physiology
MH  - Testis/growth & development/metabolism
MH  - Tissue Distribution
EDAT- 1999/07/09 00:00
MHDA- 1999/07/09 00:01
CRDT- 1999/07/09 00:00
PHST- 1999/07/09 00:00 [pubmed]
PHST- 1999/07/09 00:01 [medline]
PHST- 1999/07/09 00:00 [entrez]
AID - S0378111999002085 [pii]
AID - 10.1016/s0378-1119(99)00208-5 [doi]
PST - ppublish
SO  - Gene. 1999 Jul 8;234(2):227-37. doi: 10.1016/s0378-1119(99)00208-5.