PMID- 10395801
OWN - NLM
STAT- MEDLINE
DCOM- 19990830
LR  - 20071115
IS  - 0888-7543 (Print)
IS  - 0888-7543 (Linking)
VI  - 59
IP  - 1
DP  - 1999 Jul 1
TI  - EHD1--an EH-domain-containing protein with a specific expression pattern.
PG  - 66-76
AB  - A cDNA that is a member of the eps15 homology (EH)-domain-containing family and
      is expressed differentially in testis was isolated from mouse and human. The
      corresponding genes map to the centromeric region of mouse chromosome 19 and to
      the region of conserved synteny on human chromosome 11q13. Northern analysis
      revealed two RNA species in mouse. In addition to the high levels in testis,
      expression was noted in kidney, heart, intestine, and brain. In human, three RNA 
      species were evident. The smaller one was predominant in testis, while the
      largest species was evident in other tissues as well. The predicted protein
      sequence has an EH domain at its C-terminus, including an EF, a Ca2+ binding
      motif, and a central coiled-coil structure, as well as a nucleotide binding
      consensus site at its N-terminus. As such, it is a member of the
      EH-domain-containing protein family and was designated EHD1 (EH domain-containing
      1). In cells in tissue culture, we localized EHD1 as a green fluorescent protein 
      fusion protein, in transferrin-containing, endocytic vesicles. Immunostaining of 
      different adult mouse organs revealed major expression of EHD1 in germ cells in
      meiosis, in the testes, in adipocytes, and in specific retinal layers. Results of
      in situ hybridization to whole embryos and immunohistochemical analyses indicated
      that EHD1 expression was already noted at day 9.5 in the limb buds and pharyngeal
      arches and at day 10.5 in sclerotomes, at various elements of the branchial
      apparatus (mandible and hyoid), and in the occipital region. At day 15.5 EHD1
      expression peaked in cartilage, preceding hypertrophy and ossification, and at
      day 17.5 there was no expression in the bones. The EHD1 gene is highly conserved 
      between nematode, Drosophila, mouse, and human. Its predicted protein structure
      and cellular localization point to the possibility that EHD1 participates in
      ligand-induced endocytosis.
CI  - Copyright 1999 Academic Press.
FAU - Mintz, L
AU  - Mintz L
AD  - Department of Cell Research and Immunology, Tel-Aviv University, Ramat-Aviv,
      69978, Israel.
FAU - Galperin, E
AU  - Galperin E
FAU - Pasmanik-Chor, M
AU  - Pasmanik-Chor M
FAU - Tulzinsky, S
AU  - Tulzinsky S
FAU - Bromberg, Y
AU  - Bromberg Y
FAU - Kozak, C A
AU  - Kozak CA
FAU - Joyner, A
AU  - Joyner A
FAU - Fein, A
AU  - Fein A
FAU - Horowitz, M
AU  - Horowitz M
LA  - eng
SI  - GENBANK/AF099011
SI  - GENBANK/AF099186
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Genomics
JT  - Genomics
JID - 8800135
RN  - 0 (Carrier Proteins)
RN  - 0 (DNA, Complementary)
RN  - 0 (EHD1 protein, human)
RN  - 0 (Ehd1 protein, mouse)
RN  - 0 (Luminescent Proteins)
RN  - 0 (Recombinant Fusion Proteins)
RN  - 0 (Vesicular Transport Proteins)
RN  - 147336-22-9 (Green Fluorescent Proteins)
RN  - 63231-63-0 (RNA)
SB  - IM
MH  - Amino Acid Sequence
MH  - Animals
MH  - Blotting, Northern
MH  - COS Cells
MH  - Carrier Proteins/*genetics
MH  - Chromosome Mapping
MH  - Chromosomes/genetics
MH  - Chromosomes, Human, Pair 11/genetics
MH  - DNA, Complementary/chemistry/genetics/isolation & purification
MH  - Embryo, Mammalian/metabolism
MH  - Embryonic and Fetal Development
MH  - Endocytosis
MH  - Female
MH  - Gene Expression
MH  - Gene Expression Regulation, Developmental
MH  - Green Fluorescent Proteins
MH  - HeLa Cells
MH  - Humans
MH  - In Situ Hybridization
MH  - Luminescent Proteins/genetics
MH  - Male
MH  - Mice
MH  - Mice, Inbred ICR
MH  - Mice, Inbred Strains
MH  - Molecular Sequence Data
MH  - Muridae
MH  - RNA/genetics/metabolism
MH  - Recombinant Fusion Proteins/genetics
MH  - Sequence Alignment
MH  - Sequence Analysis, DNA
MH  - Sequence Homology, Amino Acid
MH  - Tissue Distribution
MH  - *Vesicular Transport Proteins
EDAT- 1999/07/09 00:00
MHDA- 1999/07/09 00:01
CRDT- 1999/07/09 00:00
PHST- 1999/07/09 00:00 [pubmed]
PHST- 1999/07/09 00:01 [medline]
PHST- 1999/07/09 00:00 [entrez]
AID - 10.1006/geno.1999.5800 [doi]
AID - S0888-7543(99)95800-7 [pii]
PST - ppublish
SO  - Genomics. 1999 Jul 1;59(1):66-76. doi: 10.1006/geno.1999.5800.