PMID- 10395800
OWN - NLM
STAT- MEDLINE
DCOM- 19990830
LR  - 20161124
IS  - 0888-7543 (Print)
IS  - 0888-7543 (Linking)
VI  - 59
IP  - 1
DP  - 1999 Jul 1
TI  - Cloning and genomic organization of beclin 1, a candidate tumor suppressor gene
      on chromosome 17q21.
PG  - 59-65
AB  - The beclin 1 (BECN1) gene encodes a 60-kDa coiled-coil protein that interacts
      with the prototypic apoptosis inhibitor Bcl-2. Previous studies indicate that
      beclin 1 maps to a region approximately 150 kb centromeric to BRCA1 on chromosome
      17q21 that is commonly deleted in breast, ovarian, and prostate cancer. The
      complete cDNA sequence of beclin 1 encodes a 2098-bp transcript, with a 120-bp 5'
      UTR, 1353-bp coding region, and 625-bp 3' UTR. Hybridization screening of a human
      genomic PAC library identified PAC 452O8, which contains the complete beclin 1
      gene. Determination of the exon-intron structure of beclin 1 reveals 12 exons,
      ranging from 61 to 794 bp, which extend over 12 kb of the human genome. FISH
      analysis of human breast carcinoma cell lines using PAC 452O8 as probe identified
      allelic beclin 1 deletions in 9 of 22 cell lines. Sequencing of genomic DNA from 
      10 of these cell lines revealed no mutations in coding regions or splice
      junctions. Additionally, Northern blot analysis of 11 cell lines did not identify
      any abnormalities in beclin 1 transcripts. These results indicate that human
      breast carcinoma cell lines frequently contain allelic deletions of beclin 1, but
      not beclin 1 coding mutations.
CI  - Copyright 1999 Academic Press.
FAU - Aita, V M
AU  - Aita VM
AD  - Department of Genetics and Development, Columbia University College of Physicians
      and Surgeons, New York, New York 10032, USA.
FAU - Liang, X H
AU  - Liang XH
FAU - Murty, V V
AU  - Murty VV
FAU - Pincus, D L
AU  - Pincus DL
FAU - Yu, W
AU  - Yu W
FAU - Cayanis, E
AU  - Cayanis E
FAU - Kalachikov, S
AU  - Kalachikov S
FAU - Gilliam, T C
AU  - Gilliam TC
FAU - Levine, B
AU  - Levine B
LA  - eng
SI  - GENBANK/AF139131
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Genomics
JT  - Genomics
JID - 8800135
RN  - 0 (Apoptosis Regulatory Proteins)
RN  - 0 (BECN1 protein, human)
RN  - 0 (Beclin-1)
RN  - 0 (DNA, Complementary)
RN  - 0 (Membrane Proteins)
RN  - 0 (Proteins)
RN  - 9007-49-2 (DNA)
SB  - IM
MH  - Alleles
MH  - Apoptosis Regulatory Proteins
MH  - Beclin-1
MH  - Breast Neoplasms/genetics/pathology
MH  - Chromosomes, Human, Pair 17/*genetics
MH  - Cloning, Molecular
MH  - DNA/chemistry/genetics
MH  - DNA Mutational Analysis
MH  - DNA, Complementary/chemistry/genetics
MH  - Exons
MH  - Genes, Tumor Suppressor/*genetics
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - Introns
MH  - Membrane Proteins
MH  - Molecular Sequence Data
MH  - Proteins/*genetics
MH  - Sequence Analysis, DNA
MH  - Tumor Cells, Cultured
EDAT- 1999/07/09 00:00
MHDA- 1999/07/09 00:01
CRDT- 1999/07/09 00:00
PHST- 1999/07/09 00:00 [pubmed]
PHST- 1999/07/09 00:01 [medline]
PHST- 1999/07/09 00:00 [entrez]
AID - 10.1006/geno.1999.5851 [doi]
AID - S0888-7543(99)95851-2 [pii]
PST - ppublish
SO  - Genomics. 1999 Jul 1;59(1):59-65. doi: 10.1006/geno.1999.5851.