PMID- 10395686
OWN - NLM
STAT- MEDLINE
DCOM- 19990729
LR  - 20181201
IS  - 0022-1767 (Print)
IS  - 0022-1767 (Linking)
VI  - 163
IP  - 2
DP  - 1999 Jul 15
TI  - Processing, secretion, and anti-HIV-1 activity of IL-16 with or without a signal 
      peptide in CD4+ T cells.
PG  - 906-12
AB  - CD4+ T cells transfected with the C-terminal 130 aa of human IL-16 are rendered
      resistant to HIV infection. Whether the constitutively expressed IL-16 acts
      intracellularly, extracellularly, or both is not clear. To address this question 
      and to further study the processing of IL-16, new constructs containing either
      the C-terminal 130 aa or the C-terminal 100 aa (PDZ-like motif) were constructed 
      with and without a signal peptide. Pulse-chase experiments and treatment of cells
      with brefeldin A and/or tunicamycin showed that IL-16 is secreted despite the
      absence of a signal peptide, but with a signal peptide IL-16 is processed through
      the endoplasmic reticulum-golgi pathway and is glycosylated. Cells expressing
      IL-16 linked to a signal peptide secrete considerably more IL-16 into the
      supernatant than cells expressing IL-16 without a signal peptide and are
      considerably more resistant to HIV replication. Resistance extends to almost 25
      days for cells expressing IL-16 with signal peptide as compared with only 15 days
      for cells without signal peptide. Cells expressing the C-terminal 100 aa not
      linked to a signal peptide are poor secretors of IL-16 and show little if any
      resistance to HIV. In contrast, cells expressing the C-terminal 100 aa linked to 
      a signal peptide secrete IL-16 and are resistant to HIV replication. It is
      concluded that the secretion of IL-16 is required for HIV inhibition.
FAU - Zhou, P
AU  - Zhou P
AD  - Experimental Medicine Section, Oral Infection and Immunity Branch, National
      Institute of Dental Research, Bethesda, MD 20892, USA.
FAU - Devadas, K
AU  - Devadas K
FAU - Tewari, D
AU  - Tewari D
FAU - Jegorow, A
AU  - Jegorow A
FAU - Notkins, A L
AU  - Notkins AL
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PL  - United States
TA  - J Immunol
JT  - Journal of immunology (Baltimore, Md. : 1950)
JID - 2985117R
RN  - 0 (Amino Acids)
RN  - 0 (Antiviral Agents)
RN  - 0 (Interleukin-16)
RN  - 0 (Peptide Fragments)
RN  - 0 (Protein Sorting Signals)
RN  - 0 (Recombinant Fusion Proteins)
SB  - AIM
SB  - IM
SB  - X
MH  - Amino Acid Sequence
MH  - Amino Acids/biosynthesis
MH  - Antiviral Agents/genetics/*metabolism/physiology
MH  - CD4-Positive T-Lymphocytes/immunology/virology
MH  - Extracellular Space/immunology/metabolism
MH  - Genetic Vectors/chemical synthesis
MH  - Glycosylation
MH  - HIV-1/drug effects/*immunology
MH  - Humans
MH  - Interleukin-16/genetics/*metabolism/physiology
MH  - Intracellular Fluid/immunology/metabolism
MH  - Jurkat Cells
MH  - Molecular Sequence Data
MH  - Peptide Fragments/biosynthesis/genetics/metabolism
MH  - Protein Processing, Post-Translational/*immunology
MH  - Protein Sorting Signals/genetics/*metabolism/physiology
MH  - Recombinant Fusion Proteins/biosynthesis
MH  - Transfection
MH  - Virus Replication/immunology
EDAT- 1999/07/08 00:00
MHDA- 1999/07/08 00:01
CRDT- 1999/07/08 00:00
PHST- 1999/07/08 00:00 [pubmed]
PHST- 1999/07/08 00:01 [medline]
PHST- 1999/07/08 00:00 [entrez]
AID - ji_v163n2p906 [pii]
PST - ppublish
SO  - J Immunol. 1999 Jul 15;163(2):906-12.