PMID- 10395668
OWN - NLM
STAT- MEDLINE
DCOM- 19990729
LR  - 20161124
IS  - 0022-1767 (Print)
IS  - 0022-1767 (Linking)
VI  - 163
IP  - 2
DP  - 1999 Jul 15
TI  - M1204, a novel 2',5' oligoadenylate synthetase with a ubiquitin-like extension,
      is induced during maturation of murine dendritic cells.
PG  - 760-5
AB  - A novel molecule expressed by spleen dendritic cells (DC) was isolated using a
      subtractive hybridization approach. The full-length M1204 clone has 3063 bp, with
      1415 bp spanning a single open reading frame, coding for a protein of a predicted
      size of about 50 kDa. This sequence has strong homology to 2', 5' oligoadenylate 
      synthetase and contains a ubiquitin-like domain. In Northern blot analyses the
      mRNA is strongly expressed in spleen DC, whereas, in bone marrow-derived DC, the 
      amount of mRNA increases during the maturation process. None of the other
      leukocytes nor several hemopoietic cell lines tested express this mRNA, but clear
      expression occurs in many organs, the highest levels being in thymus, lung, and
      bone marrow. In situ hybridization, combined with immunocytochemical staining of 
      tissue sections of lung and spleen, shows colocalization of M1204 with the 2A1
      and NLDC DC markers. In Western blot experiments, an antiserum raised against the
      recombinant M1204 recognizes a single band in bone marrow-derived DC and in the
      lung. The expressed oligoadenylate synthetase domain is active in synthesizing
      2',5' diadenylate, which by itself may inhibit viral protein synthesis and may
      also function as a substrate for 2',5' oligoadenylate synthetase. Since the
      oligoadenylate/RNase L system provides early protection against virus infection, 
      we hypothesize that M1204 prevents virus-induced cell death in DC.
FAU - Tiefenthaler, M
AU  - Tiefenthaler M
AD  - Department of Dermatology, Institute of Biochemistry, University of Innsbruck,
      Austria.
FAU - Marksteiner, R
AU  - Marksteiner R
FAU - Neyer, S
AU  - Neyer S
FAU - Koch, F
AU  - Koch F
FAU - Hofer, S
AU  - Hofer S
FAU - Schuler, G
AU  - Schuler G
FAU - Nussenzweig, M
AU  - Nussenzweig M
FAU - Schneider, R
AU  - Schneider R
FAU - Heufler, C
AU  - Heufler C
LA  - eng
SI  - GENBANK/AF068835
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - J Immunol
JT  - Journal of immunology (Baltimore, Md. : 1950)
JID - 2985117R
RN  - 0 (Adenine Nucleotides)
RN  - 0 (Biomarkers)
RN  - 0 (Oligoribonucleotides)
RN  - 0 (Peptide Fragments)
RN  - 0 (RNA, Messenger)
RN  - 0 (Recombinant Proteins)
RN  - 0 (Ubiquitins)
RN  - 61172-40-5 (2',5'-oligoadenylate)
RN  - EC 2.7.7.84 (2',5'-Oligoadenylate Synthetase)
SB  - AIM
SB  - IM
MH  - 2',5'-Oligoadenylate Synthetase/*biosynthesis/chemistry/genetics
MH  - Adenine Nucleotides/biosynthesis
MH  - Amino Acid Sequence
MH  - Animals
MH  - Base Sequence
MH  - Biomarkers/chemistry
MH  - Bone Marrow Cells/enzymology/metabolism
MH  - Cell Differentiation/immunology
MH  - Dendritic Cells/chemistry/enzymology/*metabolism
MH  - Enzyme Induction/immunology
MH  - Female
MH  - Male
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - Molecular Sequence Data
MH  - Oligoribonucleotides/biosynthesis
MH  - Peptide Fragments/*biosynthesis/chemistry/genetics
MH  - RNA, Messenger/biosynthesis
MH  - Recombinant Proteins/biosynthesis/isolation & purification
MH  - Sequence Homology, Amino Acid
MH  - Spleen/cytology/metabolism
MH  - Ubiquitins/*biosynthesis/chemistry/genetics
MH  - Up-Regulation/immunology
EDAT- 1999/07/08 00:00
MHDA- 1999/07/08 00:01
CRDT- 1999/07/08 00:00
PHST- 1999/07/08 00:00 [pubmed]
PHST- 1999/07/08 00:01 [medline]
PHST- 1999/07/08 00:00 [entrez]
AID - ji_v163n2p760 [pii]
PST - ppublish
SO  - J Immunol. 1999 Jul 15;163(2):760-5.