PMID- 10395639
OWN - NLM
STAT- MEDLINE
DCOM- 19990729
LR  - 20121115
IS  - 0022-1767 (Print)
IS  - 0022-1767 (Linking)
VI  - 163
IP  - 2
DP  - 1999 Jul 15
TI  - Cutting edge: combined treatment of TNF-alpha and IFN-gamma causes redistribution
      of junctional adhesion molecule in human endothelial cells.
PG  - 553-7
AB  - Proinflammatory cytokines such as TNF-alpha and IFN-gamma induce cell adhesion
      molecules in endothelial cells and promote transmigration of leukocytes across
      endothelial cells. However, when those two were administered together, leukocyte 
      transmigration paradoxically decreased. We cloned a human and bovine homologue of
      the junctional adhesion molecule (JAM), a novel molecule at the tight junction,
      and examined the effects of proinflammatory cytokines on JAM in HUVECs. The
      combined treatment of TNF-alpha plus IFN-gamma caused a disappearance of JAM from
      intercellular junctions. However, flow cytometry, cell ELISA, and subcellular
      fractionation analysis demonstrated that the amount of JAM was not reduced. This 
      suggested that JAM changed its distribution in response to proinflammatory
      cytokines. This redistribution of JAM might be involved in a decrease in
      transendothelial migration of leukocytes at inflammatory sites.
FAU - Ozaki, H
AU  - Ozaki H
AD  - Department of Geriatric Medicine, Graduate School of Medicine, Faculty of
      Medicine, Kyoto University, Japan.
FAU - Ishii, K
AU  - Ishii K
FAU - Horiuchi, H
AU  - Horiuchi H
FAU - Arai, H
AU  - Arai H
FAU - Kawamoto, T
AU  - Kawamoto T
FAU - Okawa, K
AU  - Okawa K
FAU - Iwamatsu, A
AU  - Iwamatsu A
FAU - Kita, T
AU  - Kita T
LA  - eng
SI  - GENBANK/AF111713
SI  - GENBANK/AF111714
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - J Immunol
JT  - Journal of immunology (Baltimore, Md. : 1950)
JID - 2985117R
RN  - 0 (Cell Adhesion Molecules)
RN  - 0 (Drug Combinations)
RN  - 0 (Junctional Adhesion Molecules)
RN  - 0 (Membrane Proteins)
RN  - 0 (Tumor Necrosis Factor-alpha)
RN  - 82115-62-6 (Interferon-gamma)
SB  - AIM
SB  - IM
MH  - Amino Acid Sequence
MH  - Animals
MH  - Cattle
MH  - Cell Adhesion Molecules/genetics/*metabolism
MH  - Cells, Cultured
MH  - Cloning, Molecular
MH  - Drug Combinations
MH  - Endothelium, Vascular/cytology/*immunology/*metabolism
MH  - Humans
MH  - Intercellular Junctions/metabolism
MH  - Interferon-gamma/*pharmacology
MH  - Junctional Adhesion Molecules
MH  - Membrane Proteins/immunology/metabolism
MH  - Mice
MH  - Molecular Sequence Data
MH  - Sequence Homology, Amino Acid
MH  - Subcellular Fractions/immunology/metabolism
MH  - Tumor Necrosis Factor-alpha/*pharmacology
EDAT- 1999/07/08 00:00
MHDA- 1999/07/08 00:01
CRDT- 1999/07/08 00:00
PHST- 1999/07/08 00:00 [pubmed]
PHST- 1999/07/08 00:01 [medline]
PHST- 1999/07/08 00:00 [entrez]
AID - ji_v163n2p553 [pii]
PST - ppublish
SO  - J Immunol. 1999 Jul 15;163(2):553-7.