PMID- 10394369
OWN - NLM
STAT- MEDLINE
DCOM- 19990716
LR  - 20190915
IS  - 1097-2765 (Print)
IS  - 1097-2765 (Linking)
VI  - 3
IP  - 6
DP  - 1999 Jun
TI  - Phosphatidylinositol 3-phosphate recognition by the FYVE domain.
PG  - 805-11
AB  - Recognition of phosphatidylinositol 3-phosphate (Ptdlns(3)P) is crucial for a
      broad range of cellular signaling and membrane trafficking events regulated by
      phosphoinositide (PI) 3-kinases. PtdIns(3)P binding by the FYVE domain of human
      early endosome autoantigen 1 (EEA1), a protein implicated in endosome fusion,
      involves two beta hairpins and an alpha helix. Specific amino acids, including
      those of the FYVE domain's conserved RRHHCRQCGNIF motif, contact soluble and
      micelle-embedded lipid and provide specificity for Ptdlns(3)P over Ptdlns(5)P and
      Ptdlns, as shown by heteronuclear magnetic resonance spectroscopy. Although the
      FYVE domain relies on a zinc-binding motif reminiscent of RING fingers, it is
      distinguished by ovel structural features and its ptdlns(3)P-binding site.
FAU - Kutateladze, T G
AU  - Kutateladze TG
AD  - Department of Pharmacology, University of Colorado Health Sciences Center, Denver
      80262, USA.
FAU - Ogburn, K D
AU  - Ogburn KD
FAU - Watson, W T
AU  - Watson WT
FAU - de Beer, T
AU  - de Beer T
FAU - Emr, S D
AU  - Emr SD
FAU - Burd, C G
AU  - Burd CG
FAU - Overduin, M
AU  - Overduin M
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Mol Cell
JT  - Molecular cell
JID - 9802571
RN  - 0 (Liposomes)
RN  - 0 (Membrane Proteins)
RN  - 0 (Phosphatidylinositol Phosphates)
RN  - 0 (Phosphatidylinositols)
RN  - 0 (Vesicular Transport Proteins)
RN  - 0 (early endosome antigen 1)
RN  - 0 (phosphatidylinositol 3-phosphate)
RN  - EC 2.7.1.- (Phosphatidylinositol 3-Kinases)
RN  - J41CSQ7QDS (Zinc)
SB  - IM
MH  - Amino Acid Sequence
MH  - Binding Sites
MH  - Conserved Sequence/genetics
MH  - Dimerization
MH  - Humans
MH  - Liposomes/metabolism
MH  - Membrane Proteins/analysis/*chemistry/genetics/*metabolism
MH  - Molecular Sequence Data
MH  - Molecular Weight
MH  - Mutation
MH  - Nuclear Magnetic Resonance, Biomolecular
MH  - Phosphatidylinositol 3-Kinases/metabolism
MH  - Phosphatidylinositol Phosphates/*metabolism
MH  - Phosphatidylinositols/metabolism
MH  - Protein Binding
MH  - Protein Folding
MH  - Protein Structure, Secondary
MH  - Solubility
MH  - Substrate Specificity
MH  - Vesicular Transport Proteins
MH  - Zinc/metabolism
MH  - Zinc Fingers
EDAT- 1999/07/08 00:00
MHDA- 1999/07/08 00:01
CRDT- 1999/07/08 00:00
PHST- 1999/07/08 00:00 [pubmed]
PHST- 1999/07/08 00:01 [medline]
PHST- 1999/07/08 00:00 [entrez]
AID - S1097-2765(01)80013-7 [pii]
AID - 10.1016/s1097-2765(01)80013-7 [doi]
PST - ppublish
SO  - Mol Cell. 1999 Jun;3(6):805-11. doi: 10.1016/s1097-2765(01)80013-7.