PMID- 10393926
OWN - NLM
STAT- MEDLINE
DCOM- 19990826
LR  - 20190501
IS  - 0027-8424 (Print)
IS  - 0027-8424 (Linking)
VI  - 96
IP  - 14
DP  - 1999 Jul 6
TI  - Ran-independent nuclear import of cyclin B1-Cdc2 by importin beta.
PG  - 7938-43
AB  - Mitosis is triggered in vertebrate cells by the cyclin B1-Cdc2 complex. The
      activation of this complex at the end of G2 phase is accompanied by its
      translocation from the cytoplasm to the nucleus. We used digitonin-permeabilized 
      human cells to analyze the mechanism by which cyclin B1-Cdc2 is imported into the
      nucleus. Cyclin B1-Cdc2 import was not blocked by inhibitors of the importin
      alpha-dependent import pathway or by dominant negative versions of the GTPase Ran
      or importin beta. However, the rate of cyclin B1 import was decreased by
      immunodepletion of importin beta from cytosol. Purified importin beta promoted
      cyclin B1 import in the absence of cytosol or Ran and in the presence of the
      dominant negative Ran mutant. We conclude that cyclin B1 import is mediated by an
      unusual importin beta-dependent mechanism that does not require Ran.
FAU - Takizawa, C G
AU  - Takizawa CG
AD  - Department of Physiology, University of California, San Francisco, CA 94143, USA.
FAU - Weis, K
AU  - Weis K
FAU - Morgan, D O
AU  - Morgan DO
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Proc Natl Acad Sci U S A
JT  - Proceedings of the National Academy of Sciences of the United States of America
JID - 7505876
RN  - 0 (CCNB1 protein, human)
RN  - 0 (Cyclin B)
RN  - 0 (Cyclin B1)
RN  - 0 (Karyopherins)
RN  - 0 (Nuclear Proteins)
RN  - 0 (Recombinant Proteins)
RN  - EC 2.7.11.22 (CDC2 Protein Kinase)
RN  - EC 3.6.1.- (GTP-Binding Proteins)
RN  - EC 3.6.5.2 (ran GTP-Binding Protein)
SB  - IM
MH  - Animals
MH  - CDC2 Protein Kinase/*metabolism
MH  - Cell Line
MH  - Cell Nucleus/*metabolism/ultrastructure
MH  - Cyclin B/*metabolism
MH  - Cyclin B1
MH  - GTP-Binding Proteins/metabolism
MH  - HeLa Cells
MH  - Humans
MH  - Karyopherins
MH  - Mitosis
MH  - Nuclear Proteins/*metabolism
MH  - Recombinant Proteins/metabolism
MH  - Spodoptera
MH  - Transfection
MH  - ran GTP-Binding Protein
PMC - PMC22166
EDAT- 1999/07/08 00:00
MHDA- 1999/07/08 00:01
CRDT- 1999/07/08 00:00
PHST- 1999/07/08 00:00 [pubmed]
PHST- 1999/07/08 00:01 [medline]
PHST- 1999/07/08 00:00 [entrez]
AID - 10.1073/pnas.96.14.7938 [doi]
PST - ppublish
SO  - Proc Natl Acad Sci U S A. 1999 Jul 6;96(14):7938-43. doi:
      10.1073/pnas.96.14.7938.