PMID- 10393248
OWN - NLM
STAT- MEDLINE
DCOM- 19990819
LR  - 20200728
IS  - 0378-1119 (Print)
IS  - 0378-1119 (Linking)
VI  - 234
IP  - 1
DP  - 1999 Jun 24
TI  - A serine residue in the N-terminal acidic region of rat RPB6, one of the common
      subunits of RNA polymerases, is exclusively phosphorylated by casein kinase II in
      vitro.
PG  - 139-47
AB  - RPB6 is one of the common subunits of all eukaryotic RNA polymerases and is
      indispensable for the enzyme function. Here, we isolated a rat cDNA encoding
      RPB6. It contained 127 amino acid (a.a.) residues. From alignment of RPB6
      homologues of various eukaryotes, we defined two conserved regions, i.e. an
      N-terminal acidic region and a C-terminal core. In this study, we investigated in
      vitro phosphorylation of rat RPB6 by casein kinase II (CKII), a pleiotropic
      regulator of numerous cellular proteins. Three putative CKII-phosphorylated a.a. 
      within rat RPB6 were assigned. We found that serines were phosphorylated by CKII 
      in vitro. Mutagenesis studies provided evidence that a serine at a.a. position 2 
      was exclusively phosphorylated. Finally, an RPB6-engaged in-gel kinase assay
      clarified that CKII was a prominent protein kinase in rat liver nuclear extract
      that phosphorylates RPB6. Therefore, RPB6 was implied to be phosphorylated by
      CKII in the nucleus. We postulate that the N-terminal acidic region of the RPB6
      subunit has some phosphorylation-coupled regulatory functions.
FAU - Kayukawa, K
AU  - Kayukawa K
AD  - Department of Biology, Faculty of Science, Chiba University and CREST Japan
      Science and Technology Corporation, 1-33 Yayoi-cho, Inage-ku, Chiba 263-8522,
      Japan.
FAU - Makino, Y
AU  - Makino Y
FAU - Yogosawa, S
AU  - Yogosawa S
FAU - Tamura, T
AU  - Tamura T
LA  - eng
SI  - GENBANK/AB017711
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Netherlands
TA  - Gene
JT  - Gene
JID - 7706761
RN  - 0 (DNA, Complementary)
RN  - 452VLY9402 (Serine)
RN  - EC 2.7.11.1 (Casein Kinase II)
RN  - EC 2.7.11.1 (Protein-Serine-Threonine Kinases)
RN  - EC 2.7.7.- (Polr2f protein, rat)
RN  - EC 2.7.7.6 (DNA-Directed RNA Polymerases)
SB  - IM
MH  - Amino Acid Sequence
MH  - Animals
MH  - Base Sequence
MH  - Casein Kinase II
MH  - DNA, Complementary
MH  - DNA-Directed RNA Polymerases/chemistry/*metabolism
MH  - Electrophoresis, Polyacrylamide Gel
MH  - Humans
MH  - Molecular Sequence Data
MH  - Phosphorylation
MH  - Protein-Serine-Threonine Kinases/*metabolism
MH  - Rats
MH  - Sequence Homology, Amino Acid
MH  - Serine/*metabolism
EDAT- 1999/07/07 00:00
MHDA- 1999/07/07 00:01
CRDT- 1999/07/07 00:00
PHST- 1999/07/07 00:00 [pubmed]
PHST- 1999/07/07 00:01 [medline]
PHST- 1999/07/07 00:00 [entrez]
AID - S037811199900164X [pii]
AID - 10.1016/s0378-1119(99)00164-x [doi]
PST - ppublish
SO  - Gene. 1999 Jun 24;234(1):139-47. doi: 10.1016/s0378-1119(99)00164-x.