PMID- 10393243
OWN - NLM
STAT- MEDLINE
DCOM- 19990819
LR  - 20190707
IS  - 0378-1119 (Print)
IS  - 0378-1119 (Linking)
VI  - 234
IP  - 1
DP  - 1999 Jun 24
TI  - Organization and structure of the mouse gamma-glutamyl hydrolase gene and the
      functional identification of its promoter.
PG  - 93-100
AB  - The organization and structure of the murine GH gene encoding gamma-glutamyl
      hydrolase were determined. The murine GH gene spans 24kb and was found to be
      distributed in nine exons. The intron/exon coding junctions delineated conformed 
      to the GT-AG rule. The 5' UTR and 3' UTR along with some coding sequences were
      incorporated in exons 1 and 9, respectively, whereas exons 2-8 incorporated only 
      coding sequence. A relatively GC-rich region of the genome 5' of exon 1 was
      distinctly promoter-like and encoded a number of putative cis-acting elements,
      including six Sp1 sites known to be involved in the regulation of transcription
      but no TATA sequence motif. Primer extension analysis of this region with mouse
      liver and S180 cell mRNA revealed several tsps within the region encompassing the
      Sp1 sites. Functional analysis of this 5' upstream region of sequence was carried
      out by inserting it into the pGL3 reporter gene vector for transfection into
      NIH3T3 cells. The transcription of the luciferase gene that resulted in these
      cells established the identity of this region as an active promoter for the mouse
      GH gene.
FAU - Esaki, T
AU  - Esaki T
AD  - Program in Molecular Pharmacology and Therapeutics, Memorial Sloan-Kettering
      Cancer Center, 1275 York Ave, New York, NY 10021, USA.
FAU - Masumoto, N
AU  - Masumoto N
FAU - Hayes, P
AU  - Hayes P
FAU - Chen, J
AU  - Chen J
FAU - Sirotnak, F M
AU  - Sirotnak FM
LA  - eng
SI  - GENBANK/AF090725
SI  - GENBANK/AF090726
SI  - GENBANK/AF090727
SI  - GENBANK/AF090728
SI  - GENBANK/AF090729
SI  - GENBANK/AF090730
SI  - GENBANK/AF090731
SI  - GENBANK/AF090732
SI  - GENBANK/AH008058
GR  - CA08748/CA/NCI NIH HHS/United States
GR  - CA25933/CA/NCI NIH HHS/United States
GR  - CA56517/CA/NCI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - Netherlands
TA  - Gene
JT  - Gene
JID - 7706761
RN  - 0 (DNA, Complementary)
RN  - EC 3.4.19.9 (gamma-Glutamyl Hydrolase)
SB  - IM
MH  - Animals
MH  - Base Sequence
MH  - Cloning, Molecular
MH  - DNA, Complementary
MH  - Exons
MH  - Mice
MH  - Molecular Sequence Data
MH  - *Promoter Regions, Genetic
MH  - Transcription, Genetic
MH  - gamma-Glutamyl Hydrolase/*genetics
EDAT- 1999/07/07 00:00
MHDA- 1999/07/07 00:01
CRDT- 1999/07/07 00:00
PHST- 1999/07/07 00:00 [pubmed]
PHST- 1999/07/07 00:01 [medline]
PHST- 1999/07/07 00:00 [entrez]
AID - S0378-1119(99)00174-2 [pii]
AID - 10.1016/s0378-1119(99)00174-2 [doi]
PST - ppublish
SO  - Gene. 1999 Jun 24;234(1):93-100. doi: 10.1016/s0378-1119(99)00174-2.