PMID- 10393201
OWN - NLM
STAT- MEDLINE
DCOM- 19990831
LR  - 20081120
IS  - 0261-4189 (Print)
IS  - 0261-4189 (Linking)
VI  - 18
IP  - 13
DP  - 1999 Jul 1
TI  - A human DNA editing enzyme homologous to the Escherichia coli DnaQ/MutD protein.
PG  - 3868-75
AB  - Mammalian DNA polymerases alpha and beta lack 3' exonuclease activity and are
      unable to edit errors after DNA synthesis. However, editing exonucleases can be
      functions of separate polypeptides. We isolated a widely distributed DNA-specific
      3' exonuclease from rabbit liver nuclei, sequenced tryptic peptides by mass
      spectrometry, and identified the corresponding human open reading frame. The
      protein expressed from the cloned human sequence exhibits 3' exonuclease
      activity. The human clone shares sequence homology with the editing function of
      the Escherichia coli DNA polymerase III holoenzyme, i.e., the DnaQ/MutD protein, 
      and weakly with the editing 3' exonuclease domain of eukaryotic DNA polymerase
      epsilon. The gene maps to human chromosome 3p21.2-21.3. In a reconstituted human 
      DNA repair system containing DNA polymerase beta and DNA ligase III-XRCC1,
      accurate rejoining of a 3' mismatched base residue at a single-strand break is
      dependent on addition of the exonuclease.
FAU - Hoss, M
AU  - Hoss M
AD  - Imperial Cancer Research Fund, Clare Hall Laboratories, South Mimms,
      Hertfordshire EN6 3LD, UK.
FAU - Robins, P
AU  - Robins P
FAU - Naven, T J
AU  - Naven TJ
FAU - Pappin, D J
AU  - Pappin DJ
FAU - Sgouros, J
AU  - Sgouros J
FAU - Lindahl, T
AU  - Lindahl T
LA  - eng
SI  - GENBANK/AJ243797
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - EMBO J
JT  - The EMBO journal
JID - 8208664
RN  - 0 (DNA, Single-Stranded)
RN  - 0 (Escherichia coli Proteins)
RN  - 0 (RNA, Messenger)
RN  - 0 (Recombinant Fusion Proteins)
RN  - EC 2.7.7.- (DNA Polymerase II)
RN  - EC 2.7.7.- (DNA Polymerase III)
RN  - EC 2.7.7.- (DNA Polymerase beta)
RN  - EC 2.7.7.7 (dnaQ protein, E coli)
RN  - EC 3.1.- (Exodeoxyribonucleases)
RN  - EC 3.1.11.2 (exodeoxyribonuclease III)
RN  - EC 3.1.11.5 (Exodeoxyribonuclease V)
RN  - EC 6.5.1.- (DNA Ligases)
SB  - IM
MH  - Amino Acid Sequence
MH  - Animals
MH  - Base Pair Mismatch/genetics
MH  - Catalysis
MH  - Cell Nucleus/enzymology
MH  - Chromosomes, Human, Pair 3/genetics
MH  - Cloning, Molecular
MH  - DNA Ligases/metabolism
MH  - DNA Polymerase II/chemistry/genetics
MH  - *DNA Polymerase III
MH  - DNA Polymerase beta/metabolism
MH  - DNA Repair/genetics
MH  - DNA, Single-Stranded/genetics/metabolism
MH  - Escherichia coli/enzymology/*genetics
MH  - *Escherichia coli Proteins
MH  - Exodeoxyribonuclease V
MH  - Exodeoxyribonucleases/chemistry/*genetics/isolation & purification/*metabolism
MH  - Humans
MH  - Mass Spectrometry
MH  - Molecular Sequence Data
MH  - Molecular Weight
MH  - RNA, Messenger/genetics/metabolism
MH  - Rabbits
MH  - Recombinant Fusion Proteins/biosynthesis/isolation & purification/metabolism
MH  - Sequence Homology, Amino Acid
PMC - PMC1171463
EDAT- 1999/07/07 00:00
MHDA- 1999/07/07 00:01
CRDT- 1999/07/07 00:00
PHST- 1999/07/07 00:00 [pubmed]
PHST- 1999/07/07 00:01 [medline]
PHST- 1999/07/07 00:00 [entrez]
AID - 10.1093/emboj/18.13.3868 [doi]
PST - ppublish
SO  - EMBO J. 1999 Jul 1;18(13):3868-75. doi: 10.1093/emboj/18.13.3868.