PMID- 10393184
OWN - NLM
STAT- MEDLINE
DCOM- 19990831
LR  - 20161019
IS  - 0261-4189 (Print)
IS  - 0261-4189 (Linking)
VI  - 18
IP  - 13
DP  - 1999 Jul 1
TI  - A novel RNA polymerase II-containing complex potentiates Tat-enhanced HIV-1
      transcription.
PG  - 3688-701
AB  - The HIV-1-encoded Tat protein controls transcription elongation by increasing
      processivity of RNA polymerase II (Pol II). Here, we have identified a Tat
      stimulatory activity (Tat-SF) as a novel RNA Pol II-containing complex.
      Remarkably, Tat-SF contains the previously identified Tat cofactors Tat-SF1,
      P-TEFb and hSPT5/Tat-CT1, in addition to RNA Pol II and other unidentified
      polypeptides, but none of the SRB/MED proteins or other factors found associated 
      with the previously described RNA Pol II holoenzyme complex. Tat-SF supports
      basal, Sp1-activated and Tat-activated transcription in a reconstituted system,
      and a Tat-SF-derived fraction lacking RNA Pol II can complement non-responsive
      RNA Pol II complexes for Tat-enhanced HIV-1 transcription, indicating that Tat-SF
      contains factors that are critical for Tat function. Both Tat-SF and RNA Pol II
      holoenzyme are present in HeLa nuclear extracts and each can be recruited to the 
      HIV-1 promoter. Our results indicate that Tat-SF is a Tat cofactor-containing RNA
      Pol II complex whose recruitment to the promoter provides elongation factors
      important for Tat-enhanced HIV-1 transcription following TAR RNA synthesis.
FAU - Parada, C A
AU  - Parada CA
AD  - Laboratory of Biochemistry and Molecular Biology, The Rockefeller University, New
      York, NY 10021, USA.
FAU - Roeder, R G
AU  - Roeder RG
LA  - eng
GR  - AI 37327/AI/NIAID NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - England
TA  - EMBO J
JT  - The EMBO journal
JID - 8208664
RN  - 0 (Gene Products, tat)
RN  - 0 (HTATSF1 protein, human)
RN  - 0 (Holoenzymes)
RN  - 0 (Multienzyme Complexes)
RN  - 0 (Sp1 Transcription Factor)
RN  - 0 (Trans-Activators)
RN  - 0 (Transcription Factors, TFII)
RN  - 0 (tat Gene Products, Human Immunodeficiency Virus)
RN  - EC 2.7.7.- (RNA Polymerase II)
SB  - IM
SB  - X
MH  - Cell Nucleus/enzymology/metabolism
MH  - Chromatography, Liquid
MH  - *Gene Expression Regulation, Viral
MH  - Gene Products, tat/*metabolism
MH  - Genetic Complementation Test
MH  - HIV-1/*genetics
MH  - HeLa Cells
MH  - Holoenzymes/chemistry/isolation & purification/metabolism
MH  - Humans
MH  - Molecular Weight
MH  - Multienzyme Complexes/*chemistry/isolation & purification/metabolism
MH  - Promoter Regions, Genetic/genetics
MH  - RNA Polymerase II/isolation & purification/*metabolism
MH  - Sp1 Transcription Factor/physiology
MH  - Trans-Activators/isolation & purification/metabolism
MH  - Transcription Factors, TFII/isolation & purification/metabolism
MH  - Transcription, Genetic/*genetics
MH  - tat Gene Products, Human Immunodeficiency Virus
PMC - PMC1171446
EDAT- 1999/07/07 00:00
MHDA- 1999/07/07 00:01
CRDT- 1999/07/07 00:00
PHST- 1999/07/07 00:00 [pubmed]
PHST- 1999/07/07 00:01 [medline]
PHST- 1999/07/07 00:00 [entrez]
AID - 10.1093/emboj/18.13.3688 [doi]
PST - ppublish
SO  - EMBO J. 1999 Jul 1;18(13):3688-701. doi: 10.1093/emboj/18.13.3688.