PMID- 10393183
OWN - NLM
STAT- MEDLINE
DCOM- 19990831
LR  - 20161124
IS  - 0261-4189 (Print)
IS  - 0261-4189 (Linking)
VI  - 18
IP  - 13
DP  - 1999 Jul 1
TI  - An adipogenic cofactor bound by the differentiation domain of PPARgamma.
PG  - 3676-87
AB  - Ligand activation of the nuclear receptor PPARgamma induces adipogenesis and
      increases insulin sensitivity, while activation of other PPAR isoforms (-alpha
      and -delta) induces little or no fat cell differentiation. Expression and
      activation of chimeras formed between PPARgamma and PPARdelta in fibroblasts has 
      allowed us to localize a major domain of PPARgamma responsible for adipogenesis
      to the N-terminal 138 amino acids, a region with AF-1 transcriptional activity.
      Using this region of PPARgamma as bait, we have used a yeast two-hybrid screen to
      clone a novel protein, termed PGC-2, containing a partial SCAN domain. PGC-2
      binds to and increases the transcriptional activity of PPARgamma but does not
      interact with other PPARs or most other nuclear receptors. Ectopic expression of 
      PGC-2 in preadipocytes containing endogenous PPARgamma causes a dramatic increase
      in fat cell differentiation at both the morphological and molecular levels. These
      results suggest that interactions between PGC-2, a receptor isoform-selective
      cofactor and PPARgamma contribute to the adipogenic action of this receptor.
FAU - Castillo, G
AU  - Castillo G
AD  - Dana-Farber Cancer Institute, 44 Binney Street Boston, MA 02115, USA.
FAU - Brun, R P
AU  - Brun RP
FAU - Rosenfield, J K
AU  - Rosenfield JK
FAU - Hauser, S
AU  - Hauser S
FAU - Park, C W
AU  - Park CW
FAU - Troy, A E
AU  - Troy AE
FAU - Wright, M E
AU  - Wright ME
FAU - Spiegelman, B M
AU  - Spiegelman BM
LA  - eng
SI  - GENBANK/AF220501
GR  - DK-09090/DK/NIDDK NIH HHS/United States
GR  - R01 5R37DK31405/DK/NIDDK NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - England
TA  - EMBO J
JT  - The EMBO journal
JID - 8208664
RN  - 0 (Azo Compounds)
RN  - 0 (Protein Isoforms)
RN  - 0 (RNA, Messenger)
RN  - 0 (Receptors, Cytoplasmic and Nuclear)
RN  - 0 (Receptors, Estrogen)
RN  - 0 (Recombinant Fusion Proteins)
RN  - 0 (Scand1 protein, mouse)
RN  - 0 (Transcription Factors)
RN  - G7S71FND9B (oil red O)
SB  - IM
MH  - Adipocytes/*cytology/metabolism
MH  - Amino Acid Sequence
MH  - Animals
MH  - Azo Compounds
MH  - Base Sequence
MH  - Cell Differentiation
MH  - Cell Line
MH  - Cloning, Molecular
MH  - Gene Expression Regulation
MH  - Mice
MH  - Molecular Sequence Data
MH  - Protein Binding
MH  - Protein Isoforms/chemistry/genetics/metabolism
MH  - RNA, Messenger/genetics/metabolism
MH  - Receptors, Cytoplasmic and Nuclear/agonists/*chemistry/genetics/*metabolism
MH  - Receptors, Estrogen/genetics/metabolism
MH  - Recombinant Fusion Proteins/chemistry/metabolism
MH  - Stem Cells/cytology/metabolism
MH  - Substrate Specificity
MH  - Transcription Factors/agonists/*chemistry/genetics/*metabolism
MH  - Transcription, Genetic/genetics
MH  - Transfection
MH  - Yeasts/genetics
PMC - PMC1171445
EDAT- 1999/07/07 00:00
MHDA- 1999/07/07 00:01
CRDT- 1999/07/07 00:00
PHST- 1999/07/07 00:00 [pubmed]
PHST- 1999/07/07 00:01 [medline]
PHST- 1999/07/07 00:00 [entrez]
AID - 10.1093/emboj/18.13.3676 [doi]
PST - ppublish
SO  - EMBO J. 1999 Jul 1;18(13):3676-87. doi: 10.1093/emboj/18.13.3676.