PMID- 10393182
OWN - NLM
STAT- MEDLINE
DCOM- 19990831
LR  - 20141120
IS  - 0261-4189 (Print)
IS  - 0261-4189 (Linking)
VI  - 18
IP  - 13
DP  - 1999 Jul 1
TI  - The TIM17.23 preprotein translocase of mitochondria: composition and function in 
      protein transport into the matrix.
PG  - 3667-75
AB  - We have analysed the structural organization of the TIM17.23 complex, the
      preprotein translocase of the mitochondrial inner membrane specific for protein
      targeting to the matrix. The components Tim17, Tim23 and Tim44 are present in
      this complex in equimolar amounts. A sub-complex containing Tim23 and Tim44 but
      no Tim17, or a sub-complex containing Tim23 and Tim17 but no Tim44 was not
      detected. Tim44 is peripherally associated at the matrix side. Tim44 forms dimers
      which recruit two molecules of mt-Hsp70 to the sites of protein import. A
      sequential, hand-over-hand mode of interaction of these two mt-Hsp70.Tim44
      complexes with a translocating polypeptide chain is proposed.
FAU - Moro, F
AU  - Moro F
AD  - Institut fur Physiologische Chemie der Universitat Munchen, Goethestrasse 33,
      80336 Munchen, Germany.
FAU - Sirrenberg, C
AU  - Sirrenberg C
FAU - Schneider, H C
AU  - Schneider HC
FAU - Neupert, W
AU  - Neupert W
FAU - Brunner, M
AU  - Brunner M
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - EMBO J
JT  - The EMBO journal
JID - 8208664
RN  - 0 (Carrier Proteins)
RN  - 0 (HSP70 Heat-Shock Proteins)
RN  - 0 (Membrane Proteins)
RN  - 0 (Membrane Transport Proteins)
RN  - 0 (Mitochondrial Membrane Transport Proteins)
RN  - 0 (Protein Precursors)
RN  - 0 (Repressor Proteins)
RN  - 0 (Saccharomyces cerevisiae Proteins)
RN  - 0 (TIM17 protein, S cerevisiae)
RN  - 0 (TIM23 protein, S cerevisiae)
RN  - 0 (TIM44 protein, S cerevisiae)
RN  - EC 3.4.- (Endopeptidases)
SB  - IM
MH  - Amino Acid Sequence
MH  - Animals
MH  - Biological Transport
MH  - Carrier Proteins/chemistry/*metabolism
MH  - Dimerization
MH  - Endopeptidases/metabolism
MH  - HSP70 Heat-Shock Proteins/metabolism
MH  - Intracellular Membranes/enzymology
MH  - Membrane Proteins/chemistry/*metabolism
MH  - *Membrane Transport Proteins
MH  - Mitochondria/enzymology/*metabolism
MH  - *Mitochondrial Membrane Transport Proteins
MH  - Models, Biological
MH  - Molecular Sequence Data
MH  - Molecular Weight
MH  - Osmolar Concentration
MH  - Precipitin Tests
MH  - Protein Biosynthesis
MH  - Protein Precursors/chemistry/*metabolism
MH  - *Repressor Proteins
MH  - Saccharomyces cerevisiae/cytology
MH  - *Saccharomyces cerevisiae Proteins
MH  - Sequence Alignment
MH  - Solubility
PMC - PMC1171444
EDAT- 1999/07/07 00:00
MHDA- 1999/07/07 00:01
CRDT- 1999/07/07 00:00
PHST- 1999/07/07 00:00 [pubmed]
PHST- 1999/07/07 00:01 [medline]
PHST- 1999/07/07 00:00 [entrez]
AID - 10.1093/emboj/18.13.3667 [doi]
PST - ppublish
SO  - EMBO J. 1999 Jul 1;18(13):3667-75. doi: 10.1093/emboj/18.13.3667.