PMID- 10393085
OWN - NLM
STAT- MEDLINE
DCOM- 19990908
LR  - 20201209
IS  - 0264-6021 (Print)
IS  - 0264-6021 (Linking)
VI  - 341 ( Pt 2)
DP  - 1999 Jul 15
TI  - Expression of I2PP2A, an inhibitor of protein phosphatase 2A, induces c-Jun and
      AP-1 activity.
PG  - 293-8
AB  - Transient expression of I2PP2A, a potent inhibitor of protein phosphatase 2A
      (PP2A), in HEK-293 cells increased the concentration and DNA binding of the
      proto-oncogene c-Jun. In contrast, expression of the catalytic subunit of PP2A
      (PP2AC) markedly decreased the concentration and DNA binding of c-Jun. Expression
      of I2PP2A also increased the transcriptional activity of activator protein-1, and
      this effect was diminished in a dose-dependent manner by expression of PP2AC.
      Densitometric analysis following Western blotting of extracts with antibodies
      specific for phospho-Ser63 and Ser73 suggests that the effects of I2PP2A and
      PP2AC expression might be mediated, in part, by changes in the phosphorylation of
      c-Jun at Ser63. The results indicate that I2PP2A elicits effects that are
      consistent with it acting as an inhibitor of PP2A in intact cells, and suggest
      that PP2A might exhibit site selectivity with respect to c-Jun phosphorylation.
FAU - Al-Murrani, S W
AU  - Al-Murrani SW
AD  - Department of Cellular and Molecular Physiology H166, The Pennsylvania State
      University College of Medicine, Milton S. Hershey Medical Centre, P.O. Box 850,
      Hershey, PA 17033, USA.
FAU - Woodgett, J R
AU  - Woodgett JR
FAU - Damuni, Z
AU  - Damuni Z
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Biochem J
JT  - The Biochemical journal
JID - 2984726R
RN  - 0 (Chromosomal Proteins, Non-Histone)
RN  - 0 (DNA-Binding Proteins)
RN  - 0 (Histone Chaperones)
RN  - 0 (Proteins)
RN  - 0 (SET protein, human)
RN  - 0 (Transcription Factor AP-1)
RN  - 0 (Transcription Factors)
RN  - EC 3.1.3.16 (Phosphoprotein Phosphatases)
RN  - EC 3.1.3.16 (Protein Phosphatase 2)
SB  - IM
MH  - Cell Line
MH  - Chromosomal Proteins, Non-Histone
MH  - DNA-Binding Proteins
MH  - Gene Expression Regulation
MH  - *Genes, jun
MH  - Histone Chaperones
MH  - Humans
MH  - Phosphoprotein Phosphatases/antagonists & inhibitors/*genetics
MH  - Protein Phosphatase 2
MH  - Proteins/*genetics/metabolism
MH  - Signal Transduction/*genetics
MH  - Transcription Factor AP-1/*genetics/metabolism
MH  - Transcription Factors
MH  - Transfection
PMC - PMC1220359
EDAT- 1999/07/07 00:00
MHDA- 1999/07/07 00:01
CRDT- 1999/07/07 00:00
PHST- 1999/07/07 00:00 [pubmed]
PHST- 1999/07/07 00:01 [medline]
PHST- 1999/07/07 00:00 [entrez]
PST - ppublish
SO  - Biochem J. 1999 Jul 15;341 ( Pt 2):293-8.