PMID- 10393028
OWN - NLM
STAT- MEDLINE
DCOM- 19990708
LR  - 20041117
IS  - 0146-0404 (Print)
IS  - 0146-0404 (Linking)
VI  - 40
IP  - 8
DP  - 1999 Jul
TI  - Alteration of sialyl Lewis epitope expression in pterygium.
PG  - 1631-6
AB  - PURPOSE: Mucin-related antigens are abundantly expressed by the cells of the
      normal human conjunctiva. The pattern of these antigens in pterygium, and
      especially the role of Galbeta1-3GlcNAc alpha2,3-sialyltransferase (ST3Gal III), 
      sialyltransferase necessary to build the sialyl-Le(a) (Lewis(a)) antigen, were
      studied. METHODS: Immunoperoxidase staining was performed on 28 pterygia using
      different monoclonal antibodies: anti-M1 (against the peptidic core of gastric
      mucins encoded by MUC 5AC gene), anti-Le(a)(7LE), anti-sialyl Le(a)(NS 19-9), and
      anti-Le(b)(2-25LE). A serologic Lewis determination was done in 18 patients.
      ST3Gal III sialyltransferase expression was also studied in 10 healthy
      conjunctiva and 10 pterygia by reverse transcriptase-polymerase chain reaction
      (RT-PCR). Glyceraldehyde-3-phosphate-dehydrogenase was used as an endogenous
      internal control. RESULTS: First, Le(a), sialyl Le(a), and Le(b)
      immunoreactivities either decreased or were no longer detectable in pterygium
      goblet cells as opposed to normal conjunctiva. Second, unlike in pterygium, the
      Lewis immunoreactivity, which is mainly located in the surface epithelial cells
      in the normal conjunctiva, was occasionally restricted to the epithelial cells of
      the deep layers. However, M1 mucins did show an identical pattern expression in a
      normal conjunctiva and pterygium. ST3Gal III expression was significantly lower
      in pterygium (0.20+/-0.02 AU [arbitrary units]) than in normal conjunctiva
      (0.95+/-0.12 AU). CONCLUSIONS: ST3Gal III gene is less expressed in pterygium
      than in normal conjunctiva. This observation could explain the decrease of sialyl
      Le(a) expression observed in pterygium by immunohistology.
FAU - Creuzot-Garcher, C
AU  - Creuzot-Garcher C
AD  - Department of Ophthalmology, University of Burgundy, Dijon, France.
FAU - Guerzider, V
AU  - Guerzider V
FAU - Assem, M
AU  - Assem M
FAU - Bron, A M
AU  - Bron AM
FAU - Delannoy, P
AU  - Delannoy P
FAU - Bara, J
AU  - Bara J
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Invest Ophthalmol Vis Sci
JT  - Investigative ophthalmology & visual science
JID - 7703701
RN  - 0 (ABO Blood-Group System)
RN  - 0 (Antibodies, Monoclonal)
RN  - 0 (DNA Primers)
RN  - 0 (Epitopes)
RN  - 0 (Gangliosides)
RN  - 0 (Lewis Blood-Group System)
RN  - 0 (Mucins)
RN  - 0 (RNA, Messenger)
RN  - 91847-18-6 (sialyl Le(a) ganglioside)
RN  - EC 2.4.99.- (Sialyltransferases)
RN  - EC 2.4.99.4 (beta-galactoside alpha-2,3-sialyltransferase)
SB  - IM
MH  - ABO Blood-Group System/metabolism
MH  - Adult
MH  - Aged
MH  - Aged, 80 and over
MH  - Antibodies, Monoclonal
MH  - Conjunctiva/cytology/metabolism
MH  - DNA Primers/chemistry
MH  - Epitopes/*metabolism
MH  - Gangliosides/metabolism
MH  - Humans
MH  - Immunoenzyme Techniques
MH  - Lewis Blood-Group System/*metabolism
MH  - Middle Aged
MH  - Mucins/*metabolism
MH  - Phenotype
MH  - Pterygium/genetics/*metabolism/pathology
MH  - RNA, Messenger/analysis
MH  - Reverse Transcriptase Polymerase Chain Reaction
MH  - Sialyltransferases/genetics/*metabolism
EDAT- 1999/07/07 00:00
MHDA- 1999/07/07 00:01
CRDT- 1999/07/07 00:00
PHST- 1999/07/07 00:00 [pubmed]
PHST- 1999/07/07 00:01 [medline]
PHST- 1999/07/07 00:00 [entrez]
PST - ppublish
SO  - Invest Ophthalmol Vis Sci. 1999 Jul;40(8):1631-6.