PMID- 10391944
OWN - NLM
STAT- MEDLINE
DCOM- 19990805
LR  - 20200204
IS  - 0021-9258 (Print)
IS  - 0021-9258 (Linking)
VI  - 274
IP  - 28
DP  - 1999 Jul 9
TI  - Ig-hepta, a novel member of the G protein-coupled hepta-helical receptor (GPCR)
      family that has immunoglobulin-like repeats in a long N-terminal extracellular
      domain and defines a new subfamily of GPCRs.
PG  - 19957-64
AB  - A novel member of the G protein-coupled receptor (GPCR) family was cloned and
      characterized, which is unique, among the members, in its long extracellular
      domain comprising Ig-like repeats and in its high expression predominantly in the
      lung. The clone (Ig-Hepta) was first identified as a polymerase chain reaction
      product generated with primers designed to amplify secretin receptor family
      members including the parathyroid hormone-related peptide receptors. Analysis of 
      the open reading frame of cDNAs isolated from a rat lung cDNA library indicated
      that Ig-Hepta is a protein of 1389 amino acid residues and has two Ig-like
      repeats in the N-terminal extracellular domain (exodomain) of 1053 amino acid
      residues and 7 transmembrane spans in the C-terminal region. Northern blot
      analysis revealed very high expression of its mRNA in the lung and low but
      detectable levels in the kidney and heart. The mRNA expression in the lung was
      found to be strongly induced postnatally. Biochemical analysis indicated that
      Ig-Hepta is a highly glycosylated protein and exists as a disulfide-linked dimer.
      Immunohistochemistry on rat lung and kidney sections revealed dense localization 
      of Ig-Hepta in alveolar walls and intercalated cells in the collecting duct,
      respectively, suggesting a role in the regulation of acid-base balance. Ig-Hepta 
      defines a new subfamily of GPCRs.
FAU - Abe, J
AU  - Abe J
AD  - Department of Biological Sciences, Tokyo Institute of Technology, 4259
      Nagatsuta-cho, Midoriku, Yokohama 226-8501, Japan.
FAU - Suzuki, H
AU  - Suzuki H
FAU - Notoya, M
AU  - Notoya M
FAU - Yamamoto, T
AU  - Yamamoto T
FAU - Hirose, S
AU  - Hirose S
LA  - eng
SI  - GENBANK/AB019120
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - J Biol Chem
JT  - The Journal of biological chemistry
JID - 2985121R
RN  - 0 (Adgrf5 protein, rat)
RN  - 0 (Immunoglobulins)
RN  - 0 (RNA, Messenger)
RN  - 0 (Receptors, Cell Surface)
RN  - 0 (Receptors, G-Protein-Coupled)
RN  - EC 3.6.1.- (GTP-Binding Proteins)
SB  - IM
MH  - Amino Acid Sequence
MH  - Animals
MH  - COS Cells
MH  - Cloning, Molecular
MH  - GTP-Binding Proteins/chemistry/*genetics
MH  - Glycosylation
MH  - Immunoglobulins/*chemistry
MH  - Kidney/metabolism
MH  - Lung/metabolism
MH  - Molecular Sequence Data
MH  - Phylogeny
MH  - RNA, Messenger/metabolism
MH  - Rats
MH  - Rats, Wistar
MH  - Receptors, Cell Surface/chemistry/*genetics
MH  - *Receptors, G-Protein-Coupled
MH  - Repetitive Sequences, Nucleic Acid
MH  - Sequence Alignment
MH  - Transfection
EDAT- 1999/07/03 00:00
MHDA- 1999/07/03 00:01
CRDT- 1999/07/03 00:00
PHST- 1999/07/03 00:00 [pubmed]
PHST- 1999/07/03 00:01 [medline]
PHST- 1999/07/03 00:00 [entrez]
AID - 10.1074/jbc.274.28.19957 [doi]
PST - ppublish
SO  - J Biol Chem. 1999 Jul 9;274(28):19957-64. doi: 10.1074/jbc.274.28.19957.