PMID- 10391924
OWN - NLM
STAT- MEDLINE
DCOM- 19990805
LR  - 20190508
IS  - 0021-9258 (Print)
IS  - 0021-9258 (Linking)
VI  - 274
IP  - 28
DP  - 1999 Jul 9
TI  - Cypher, a striated muscle-restricted PDZ and LIM domain-containing protein, binds
      to alpha-actinin-2 and protein kinase C.
PG  - 19807-13
AB  - We have cloned and characterized a novel striated muscle-restricted protein
      (Cypher) that has two mRNA splice variants, designated Cypher1 and Cypher2. Both 
      proteins contain an amino-terminal PDZ domain. Cypher1, but not Cypher2, contains
      three carboxyl-terminal LIM domains and an amino acid repeat sequence that
      exhibits homology to a repeat sequence found in the largest subunit of RNA
      polymerase II. cypher1 and cypher2 mRNAs exhibited identical expression patterns.
      Both are exclusively expressed in cardiac and striated muscle in embryonic and
      adult stages. By biochemical assays, we have demonstrated that Cypher1 and
      Cypher2 bind to alpha-actinin-2 via their PDZ domains. This interaction has been 
      further confirmed by immunohistochemical studies that demonstrated
      co-localization of Cypher and alpha-actinin at the Z-lines of cardiac muscle. We 
      have also found that Cypher1 binds to protein kinase C through its LIM domains.
      Phosphorylation of Cypher by protein kinase C has demonstrated the functional
      significance of this interaction. Together, our data suggest that Cypher1 may
      function as an adaptor in striated muscle to couple protein kinase C-mediated
      signaling, via its LIM domains, to the cytoskeleton (alpha-actinin-2) through its
      PDZ domain.
FAU - Zhou, Q
AU  - Zhou Q
AD  - Department of Medicine, UCSD-Salk Program in Molecular Medicine, University of
      California at San Diego, School of Medicine, La Jolla, California 92093-0613,
      USA.
FAU - Ruiz-Lozano, P
AU  - Ruiz-Lozano P
FAU - Martone, M E
AU  - Martone ME
FAU - Chen, J
AU  - Chen J
LA  - eng
SI  - GENBANK/AA114379
SI  - GENBANK/AF114378
SI  - GENBANK/AF114379
GR  - RR04050/RR/NCRR NIH HHS/United States
PT  - Journal Article
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - J Biol Chem
JT  - The Journal of biological chemistry
JID - 2985121R
RN  - 0 (Adaptor Proteins, Signal Transducing)
RN  - 0 (Carrier Proteins)
RN  - 0 (Homeodomain Proteins)
RN  - 0 (LDB3 protein, human)
RN  - 0 (LIM Domain Proteins)
RN  - 0 (Ldb3 protein, mouse)
RN  - 0 (Muscle Proteins)
RN  - 0 (RNA, Messenger)
RN  - 11003-00-2 (Actinin)
RN  - EC 2.7.11.13 (Protein Kinase C)
SB  - IM
MH  - Actinin/*metabolism
MH  - Adaptor Proteins, Signal Transducing
MH  - Alternative Splicing
MH  - Amino Acid Sequence
MH  - Animals
MH  - *Carrier Proteins
MH  - Cloning, Molecular
MH  - Gene Expression Regulation, Developmental
MH  - *Homeodomain Proteins
MH  - Humans
MH  - LIM Domain Proteins
MH  - Mice
MH  - Molecular Sequence Data
MH  - Muscle Proteins/chemistry/*genetics
MH  - Muscle, Skeletal/metabolism
MH  - Myocardium/metabolism
MH  - Phosphorylation
MH  - Protein Binding
MH  - Protein Kinase C/*metabolism
MH  - RNA, Messenger/genetics
MH  - Sequence Alignment
EDAT- 1999/07/03 00:00
MHDA- 1999/07/03 00:01
CRDT- 1999/07/03 00:00
PHST- 1999/07/03 00:00 [pubmed]
PHST- 1999/07/03 00:01 [medline]
PHST- 1999/07/03 00:00 [entrez]
AID - 10.1074/jbc.274.28.19807 [doi]
PST - ppublish
SO  - J Biol Chem. 1999 Jul 9;274(28):19807-13. doi: 10.1074/jbc.274.28.19807.