PMID- 10391131
OWN - NLM
STAT- MEDLINE
DCOM- 19990803
LR  - 20190818
IS  - 0300-8177 (Print)
IS  - 0300-8177 (Linking)
VI  - 194
IP  - 1-2
DP  - 1999 Apr
TI  - Molecular cloning and developmental expression of rat glycogenin in cardiac
      tissue.
PG  - 117-23
AB  - Glycogenin is a self-glycosylating protein required to initiate glycogen
      biosynthesis. Utilizing the differential display technique to analyze changes in 
      gene expression during early postnatal cardiac development, we have isolated and 
      cloned a 484 bp cDNA fragment that corresponds to the 3' end of rat glycogenin.
      Northern blot analysis on neonatal cardiac tissues demonstrated hybridization to 
      a 1.7-1.8 kb transcript, which was highly expressed at 3 days and at
      progressively reduced levels at 1, 2, 3 and 4 weeks of age. A 1624 bp fragment of
      rat glycogenin was cloned by RT-PCR that includes a 1002 bp open reading frame
      encoding a 333 amino acid protein. At the nucleotide level, rat glycogenin
      exhibited 87.2 and 83.6% identity with human and rabbit glycogenin over the open 
      reading frame. The deduced amino acid sequence showed 86.7 and 83.4% identity
      with human and rabbit sequences, respectively. Given the significance of
      glycogenin in glycogen biosynthesis, the results of this study suggest a possible
      molecular basis for the regulation of glycogen during early postnatal cardiac
      development. In addition, the nucleotide and amino acid sequences of rat
      glycogenin may be used to investigate the physiological and pathophysiological
      roles of glycogenin in rat tissues.
FAU - Pak, B J
AU  - Pak BJ
AD  - Department of Anatomy and Cell Biology, Queen's University, Kingston, Ontario,
      Canada.
FAU - Sangaralingham, S J
AU  - Sangaralingham SJ
FAU - Pang, S C
AU  - Pang SC
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Netherlands
TA  - Mol Cell Biochem
JT  - Molecular and cellular biochemistry
JID - 0364456
RN  - 0 (DNA, Complementary)
RN  - 0 (Glycoproteins)
RN  - 0 (glycogenin)
RN  - EC 2.4.1.- (Glucosyltransferases)
SB  - IM
MH  - Amino Acid Sequence
MH  - Animals
MH  - Base Sequence
MH  - Cloning, Molecular
MH  - DNA, Complementary
MH  - Female
MH  - *Gene Expression Regulation, Developmental
MH  - Glucosyltransferases
MH  - Glycoproteins/*genetics
MH  - Humans
MH  - Molecular Sequence Data
MH  - Myocardium/*metabolism
MH  - Pregnancy
MH  - Rats
MH  - Rats, Wistar
MH  - Sequence Homology, Amino Acid
EDAT- 1999/07/03 00:00
MHDA- 1999/07/03 00:01
CRDT- 1999/07/03 00:00
PHST- 1999/07/03 00:00 [pubmed]
PHST- 1999/07/03 00:01 [medline]
PHST- 1999/07/03 00:00 [entrez]
AID - 10.1023/a:1006924016809 [doi]
PST - ppublish
SO  - Mol Cell Biochem. 1999 Apr;194(1-2):117-23. doi: 10.1023/a:1006924016809.