PMID- 10390538
OWN - NLM
STAT- MEDLINE
DCOM- 19990903
LR  - 20190501
IS  - 0305-1048 (Print)
IS  - 0305-1048 (Linking)
VI  - 27
IP  - 14
DP  - 1999 Jul 15
TI  - A human Raf-responsive zinc-finger protein that binds to divergent sequences.
PG  - 2947-56
AB  - LZ321, a human liver cDNA, encodes a protein that bound to a Drosophila tramtrack
      binding site, GGTCCT. The sequence of LZ321 matched that of RREB1, a
      transcription factor that bound to a Ras responsive element (RRE) very different 
      from the sequence with which we isolated LZ321. We therefore examined the binding
      of RREB1/LZ321 to different ligands. It bound to the GGTCCT-containing ligand and
      to the RRE with similar affinities (Kd50-60 nM), but did not bind to a consensus 
      RREB1 binding site. The RREB1/LZ321 protein contains four C2H2zinc-fingers, the
      C-terminal two of which retained specific DNA binding to both ligands. A trimer
      of the GGTCCT site functioned as an enhancer in both CV-1 and H4IIE-C3 cells.
      Thus RREB1/LZ321 could function as a downstream activator in the Ras-Raf
      signaling pathway through different cis -acting elements. A longer human protein,
      Finb, contains RREB1/LZ321, and there are close homologs in both chicken and
      Drosophila, arguing that it plays important roles. The ability of transcription
      factors such as RREB1/LZ321 to bind diverse sequences gives them the potential to
      regulate previously unsuspected genes.
FAU - Zhang, L
AU  - Zhang L
AD  - Department of Biochemistry and Molecular Biology, Indiana University School of
      Medicine, Indianapolis, IN 46202-5122, USA.
FAU - Zhao, J
AU  - Zhao J
FAU - Edenberg, H J
AU  - Edenberg HJ
LA  - eng
SI  - GENBANK/AF072825
SI  - GENBANK/AF072826
GR  - AA06460/AA/NIAAA NIH HHS/United States
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - England
TA  - Nucleic Acids Res
JT  - Nucleic acids research
JID - 0411011
RN  - 0 (DNA-Binding Proteins)
RN  - 0 (Drosophila Proteins)
RN  - 0 (RREB1 protein, human)
RN  - 0 (Repressor Proteins)
RN  - 0 (Transcription Factors)
RN  - 0 (ttk protein, Drosophila)
RN  - 9007-49-2 (DNA)
RN  - EC 2.7.11.1 (Proto-Oncogene Proteins c-raf)
RN  - EC 3.6.5.2 (ras Proteins)
SB  - IM
MH  - Animals
MH  - Base Sequence
MH  - Binding Sites
MH  - Cell Line
MH  - Cloning, Molecular
MH  - DNA/genetics/*metabolism
MH  - DNA Footprinting
MH  - DNA-Binding Proteins/chemistry/genetics/*metabolism
MH  - *Drosophila Proteins
MH  - Drosophila melanogaster/genetics
MH  - Enhancer Elements, Genetic/genetics
MH  - Humans
MH  - Liver/metabolism
MH  - Molecular Sequence Data
MH  - Proto-Oncogene Proteins c-raf/*physiology
MH  - Repressor Proteins/genetics
MH  - Response Elements/*genetics
MH  - Signal Transduction
MH  - Transcription Factors/chemistry/genetics/*metabolism
MH  - Transcriptional Activation
MH  - Transfection
MH  - *Zinc Fingers
MH  - ras Proteins/physiology
PMC - PMC148511
EDAT- 1999/07/03 00:00
MHDA- 1999/07/03 00:01
CRDT- 1999/07/03 00:00
PHST- 1999/07/03 00:00 [pubmed]
PHST- 1999/07/03 00:01 [medline]
PHST- 1999/07/03 00:00 [entrez]
AID - gkc457 [pii]
AID - 10.1093/nar/27.14.2947 [doi]
PST - ppublish
SO  - Nucleic Acids Res. 1999 Jul 15;27(14):2947-56. doi: 10.1093/nar/27.14.2947.