PMID- 10390358
OWN - NLM
STAT- MEDLINE
DCOM- 19990726
LR  - 20190619
IS  - 0036-8075 (Print)
IS  - 0036-8075 (Linking)
VI  - 285
IP  - 5424
DP  - 1999 Jul 2
TI  - Paracellin-1, a renal tight junction protein required for paracellular Mg2+
      resorption.
PG  - 103-6
AB  - Epithelia permit selective and regulated flux from apical to basolateral surfaces
      by transcellular passage through cells or paracellular flux between cells. Tight 
      junctions constitute the barrier to paracellular conductance; however, little is 
      known about the specific molecules that mediate paracellular permeabilities.
      Renal magnesium ion (Mg2+) resorption occurs predominantly through a paracellular
      conductance in the thick ascending limb of Henle (TAL). Here, positional cloning 
      has identified a human gene, paracellin-1 (PCLN-1), mutations in which cause
      renal Mg2+ wasting. PCLN-1 is located in tight junctions of the TAL and is
      related to the claudin family of tight junction proteins. These findings provide 
      insight into Mg2+ homeostasis, demonstrate the role of a tight junction protein
      in human disease, and identify an essential component of a selective paracellular
      conductance.
FAU - Simon, D B
AU  - Simon DB
AD  - Howard Hughes Medical Institute, Department of Genetics, Yale University School
      of Medicine, New Haven, CT 06510, USA.
FAU - Lu, Y
AU  - Lu Y
FAU - Choate, K A
AU  - Choate KA
FAU - Velazquez, H
AU  - Velazquez H
FAU - Al-Sabban, E
AU  - Al-Sabban E
FAU - Praga, M
AU  - Praga M
FAU - Casari, G
AU  - Casari G
FAU - Bettinelli, A
AU  - Bettinelli A
FAU - Colussi, G
AU  - Colussi G
FAU - Rodriguez-Soriano, J
AU  - Rodriguez-Soriano J
FAU - McCredie, D
AU  - McCredie D
FAU - Milford, D
AU  - Milford D
FAU - Sanjad, S
AU  - Sanjad S
FAU - Lifton, R P
AU  - Lifton RP
LA  - eng
SI  - GENBANK/AF152101
GR  - F.1/Telethon/Italy
GR  - TGM06S01/Telethon/Italy
GR  - R01DK51696/DK/NIDDK NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Science
JT  - Science (New York, N.Y.)
JID - 0404511
RN  - 0 (Claudins)
RN  - 0 (Membrane Proteins)
RN  - 0 (claudin 16)
RN  - I38ZP9992A (Magnesium)
RN  - SY7Q814VUP (Calcium)
SB  - IM
CIN - Science. 1999 Jul 2;285(5424):62. PMID: 10428705
MH  - Amino Acid Sequence
MH  - Calcium/urine
MH  - Chromosomes, Human, Pair 3/genetics
MH  - Claudins
MH  - Cloning, Molecular
MH  - Female
MH  - Genes, Recessive
MH  - Homeostasis
MH  - Humans
MH  - Kidney Diseases/*genetics/metabolism
MH  - Kidney Tubules/chemistry
MH  - Loop of Henle/chemistry/*metabolism
MH  - Magnesium/blood/*metabolism
MH  - Magnesium Deficiency/*genetics/metabolism
MH  - Male
MH  - Membrane Proteins/analysis/chemistry/genetics/*physiology
MH  - Molecular Sequence Data
MH  - Mutation
MH  - Pedigree
MH  - Physical Chromosome Mapping
MH  - Tight Junctions/*metabolism
EDAT- 1999/07/03 00:00
MHDA- 1999/07/03 00:01
CRDT- 1999/07/03 00:00
PHST- 1999/07/03 00:00 [pubmed]
PHST- 1999/07/03 00:01 [medline]
PHST- 1999/07/03 00:00 [entrez]
AID - 7616 [pii]
AID - 10.1126/science.285.5424.103 [doi]
PST - ppublish
SO  - Science. 1999 Jul 2;285(5424):103-6. doi: 10.1126/science.285.5424.103.